Phospho-TAB1 (Ser438) Antibody - #AF8324

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Product: Phospho-TAB1 (Ser438) Antibody
Catalog: AF8324
Description: Rabbit polyclonal antibody to Phospho-TAB1 (Ser438)
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 55 kDa; 55kD(Calculated).
Uniprot: Q15750
RRID: AB_2840386

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-TAB1 (Ser438) Antibody detects endogenous levels of TAB1 only when phosphorylated at Ser438.
RRID:
AB_2840386
Cite Format: Affinity Biosciences Cat# AF8324, RRID:AB_2840386.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2310012M03Rik; 3'-Tab1; MAP3K7IP 1; MAP3K7IP1; MGC57664; Mitogen activated protein kinase kinase kinase 7 interacting protein 1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TAB 1; TAB1; TAB1_HUMAN; TAK1 binding protein 1; TAK1-binding protein 1; TGF beta activated kinase 1 binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TGF-beta-activated kinase 1-binding protein 1; Transforming growth factor beta activated kinase binding protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from human TAB1 around the phosphorylation site of Ser438.

Uniprot:

Q15750(TAB1_HUMAN) >>Visit HPA database.

Gene(ID):
TAB1(10454)
Expression:
Q15750 TAB1_HUMAN:

Ubiquitous.

Sequence:
MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKTLEREISGGAMAVVAVLLNNKLYVANVGTNRALLCKSTVDGLQVTQLNVDHTTENEDELFRLSQLGLDAGKIKQVGIICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERARFCPRHEDMTLLVRNFGYPLGEMSQPTPSPAPAAGGRVYPVSVPYSSAQSTSKTSVTLSLVMPSQGQMVNGAHSASTLDEATPTLTNQSPTLTLQSTNTHTQSSSSSSDGGLFRSRPAHSLPPGEDGRVEPYVDFAEFYRLWSVDHGEQSVVTAP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
67
Bovine
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.

PTMs:

Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.

Tissue Specificity:

Ubiquitous.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. Cell discovery, 2022 (PubMed: 35945223) [IF=33.5]

Application: WB    Species: Human    Sample: A549 cells

Fig. 2 TAB1 interacts with GFAT1 in a Ser438 phosphorylation-dependent manner. a TAB1 could interact with GFAT1. A549 cells with stable expression of Flag-GFAT1 were cultured for 8 h under glucose deprivation. Immunoprecipitation and immunoblotting analyses were performed using the indicated antibodies. Silver staining analysis of the immunoprecipitates was performed. b TAB1–GFAT1 interaction was phosphorylation-dependent. A549 cells were cultured for 8 h under glucose deprivation. Immunoprecipitation and immunoblotting analyses were performed using the indicated antibodies. c TAB1 phosphorylation mediated TAB1–GFAT1 interaction. The indicated purified GST-GFAT1 or His-TAB1 protein was mixed with cell lysates from A549 cells cultured for 8 h under glucose deprivation. Pull-down and immunoblotting analyses were performed using the indicated antibodies. d JNK/p38 mediated TAB1–GFAT1 interaction. A549 cells were pretreated with Compound C (10 μM), SP600125 (20 μM), and SB203580 (10 μM) for 1 h before being cultured for 8 h under glucose deprivation. Immunoprecipitation and immunoblotting analyses were performed using the indicated antibodies. e Ser438 of TAB1 is evolutionarily conserved in the indicated species (upper panel). TAB1 Ser438 phosphorylation mediated TAB1–GFAT1 interaction. A549 cells with transient expression of WT TAB1, TAB1-S423A, TAB1-T431A or TAB1-S438A were cultured for 8 h under glucose deprivation. Immunoprecipitation and immunoblotting analyses were performed using the indicated antibodies (lower panel). f His-TAB1 Ser438 could be phosphorylated by p38α or JNK1. Purified His-TAB1 proteins were subjected to in vitro kinase assay. g, h In vitro binding of His-TAB1 and GST-GFAT1. Purified His-TAB1 WT and His-TAB1-S438A protein with phosphorylation by p38α (g) or JNK1 (h) was mixed with purified GST-GFAT1. Pull-down and immunoblotting analyses were performed using the indicated antibodies. i Expression of rTAB1-S438A inhibited p38 activation at late time points. A549 cells with depleted TAB1 and reconstituted expression of WT rTAB1 or rTAB1-S438A were cultured for indicated time under glucose deprivation. Immunoblotting analyses were performed using the indicated antibodies. In i, the values are presented as means ± SEM, n = 3; *P 

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