Product: Phospho-JAK1 (Tyr1022)[Tyr1034] Antibody
Catalog: AF2012
Description: Rabbit polyclonal antibody to Phospho-JAK1 (Tyr1022)[Tyr1034]
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 130kDa; 133kD(Calculated).
Uniprot: P23458
RRID: AB_2834437

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
Phospho-JAK1 (Tyr1022)[Tyr1034] Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1034, which site historically referenced as Tyr1022.
RRID:
AB_2834437
Cite Format: Affinity Biosciences Cat# AF2012, RRID:AB_2834437.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1;

Immunogens

Immunogen:

A synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1034.

Uniprot:
Gene(ID):
Expression:
P23458 JAK1_HUMAN:

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

Description:
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain.
Sequence:
MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Xenopus
91
Zebrafish
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor.

PTMs:

Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.

Ubiquitinated by RNF125; leading to its degradation by the proteasome.

Subcellular Location:

Endomembrane system>Peripheral membrane protein.
Note: Wholly intracellular, possibly membrane associated.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.

Family&Domains:

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

The FERM domain mediates interaction with JAKMIP1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

References

1). Reactive oxygen species (ROS)-mediated M1 macrophage-dependent nanomedicine remodels inflammatory microenvironment for osteoarthritis recession. Bioactive materials, 2023 (PubMed: 38162513) [IF=18.9]

Application: WB    Species: Mouse    Sample: RAW 264.7 cells

Fig. 9. Transcriptomic analysis of BTZ treatment in LPS-induced inflammatory response. (A) Volcano plot representation of differentially expressed genes between PBS and BTZ group (adjusted 1.5 fold, p-value

2). A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis. Small (Weinheim an der Bergstrasse, Germany), 2024 (PubMed: 39679768) [IF=13.0]

3). Aligned electrospun poly(l-lactide) nanofibers facilitate wound healing by inhibiting macrophage M1 polarization via the JAK-STAT and NF-κB pathways. JOURNAL OF NANOBIOTECHNOLOGY, 2022 (PubMed: 35883095) [IF=10.2]

Application: WB    Species: Mice    Sample:

Fig. 3 The underlying mechanism by which aligned fibers affected macrophage polarization. A Venn diagram showing differentially expressed genes. B KEGG pathway analysis between the A20 and R20 groups. C Heatmap of differentially expressed genes among the three groups. D Heatmap of macrophage polarization-related genes between the A20 and R20 groups. E Volcano diagram of differentially expressed genes. F Western blot analysis of the NF-κB signaling pathway. G Immunofluorescence staining showing the nuclear translocation of NF-κB p65. The nucleus is stained blue, and NF-κB p65 protein is stained red. H Western blot images and semiquantitative analysis of the JAK-STAT signaling pathway (*p < 0.05, **p < 0.01, n = 3)

4). Graveoline attenuates D-GalN/LPS-induced acute liver injury via inhibition of JAK1/STAT3 signaling pathway. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 39018876) [IF=6.9]

5). XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36791564) [IF=6.9]

6). Leptin promotes angiogenesis via pericyte STAT3 pathway upon intracerebral hemorrhage. Cells, 2022 (PubMed: 36078162) [IF=6.0]

7). A novel fluorinated triazole derivative suppresses macrophage activation and alleviates experimental colitis via a Twist1-dependent pathway. BIOCHEMICAL PHARMACOLOGY, 2018 (PubMed: 30028990) [IF=5.3]

8). Linoleyl acetate and mandenol alleviate HUA-induced ED via NLRP3 inflammasome and JAK2/STAT3 signalling conduction in rats. Journal of cellular and molecular medicine, 2024 (PubMed: 39245800) [IF=5.3]

9). Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2020 (PubMed: 32233952) [IF=5.0]

Application: WB    Species: Mouse    Sample: bone marrow-derived mesenchymal stem cells (BMSCs)

Fig. 6. Involvement of the JAK1/STAT3 pathway and the paracrine effects of irradiation-induced senescent BMSCs on osteoblasts osteogenic differentiation. A-B:Activation of JAK1/STAT3 pathway after irradiation of 10Gy, as shown in western blot analyses of p-JAK1, T-STAT3 and p-STAT3, while 0.8μM JAK1 inhibitor intervention could effectively inhibite JAK1/STAT3 pathway, and the downstream p-STAT3 expression was more significantly blocked. C: ELISA results show the upregulated secretion of IL-6, IL-8 and MMP9 in the supernatant collected 72h after Downloaded from journals.physiology.org/journal/ajpcell at Lunds Univ Medicinska Fak Biblio (130.235.066.010) on April 8, 2020.10Gy-irradiation and suppressed secretion after adding 0.8μM JAK1 inhibitor following irradiation. D-G: Representative images and quantitative analysis of ALP activity and mineralized nodule area of osteoblasts co-cultured with different types of CM from irradiated BMSCs (Con CM, Con/JAKi CM, IRIS CM and IRIS/JAKi CM).Magnification, ×100. H-I: Relative mRNA and protein expression levels of ALP and OC of osteoblasts co-cultured with four types of CM from irradiated BMSCs. All data were analyzed from three independent experiments. P values were calculated by Student’s t-test and one-way ANOVA analysis. Results are presented as mean ± SD.*p < 0.01, **p < 0.01, ***p < 0.001. ALP: alkaline phosphatase; OC: osteocalcin; Con CM: CM from control BMSCs; Con/JAKi CM: CM from control BMSCs with JAKi intervention; IRIS CM: CM from irradiation-induced senescent BMSCs; IRIS/JAKi CM: CM from irradiation-induced senescent BMSCs with JAKi intervention. SD:standard deviation. Scale bar: 100μm (D, F).

10). DACH1 regulates macrophage activation and tumour progression in hypopharyngeal squamous cell carcinoma. Immunology, 2023 (PubMed: 37243970) [IF=4.9]

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