ITGA6 Antibody - #DF8323
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| Product: | ITGA6 Antibody |
| Catalog: | DF8323 |
| Description: | Rabbit polyclonal antibody to ITGA6 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 127 kDa; 127kD(Calculated). |
| Uniprot: | P23229 |
| RRID: | AB_2841599 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8323, RRID:AB_2841599.
Fold/Unfold
Alpha6p; CD49 antigen-like family member F; CD49f; Integrin alpha6B; Integrin, alpha 6; ITA6_HUMAN; ITGA6; ITGA6B; Processed integrin alpha-6; VLA 6; VLA-6;
Immunogens
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Processed integrin alpha-6: Expressed at low levels in normal prostate tissue with elevated levels in prostate cancer tissue (at protein level) (PubMed:15023541).
- P23229 ITA6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAGQLCLLYLSAGLLSRLGAAFNLDTREDNVIRKYGDPGSLFGFSLAMHWQLQPEDKRLLLVGAPRAEALPLQRANRTGGLYSCDITARGPCTRIEFDNDADPTSESKEDQWMGVTVQSQGPGGKVVTCAHRYEKRQHVNTKQESRDIFGRCYVLSQNLRIEDDMDGGDWSFCDGRLRGHEKFGSCQQGVAATFTKDFHYIVFGAPGTYNWKGIVRVEQKNNTFFDMNIFEDGPYEVGGETEHDESLVPVPANSYLGLLFLTSVSYTDPDQFVYKTRPPREQPDTFPDVMMNSYLGFSLDSGKGIVSKDEITFVSGAPRANHSGAVVLLKRDMKSAHLLPEHIFDGEGLASSFGYDVAVVDLNKDGWQDIVIGAPQYFDRDGEVGGAVYVYMNQQGRWNNVKPIRLNGTKDSMFGIAVKNIGDINQDGYPDIAVGAPYDDLGKVFIYHGSANGINTKPTQVLKGISPYFGYSIAGNMDLDRNSYPDVAVGSLSDSVTIFRSRPVINIQKTITVTPNRIDLRQKTACGAPSGICLQVKSCFEYTANPAGYNPSISIVGTLEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLKRQKQKVCMEETLWLQDNIRDKLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSDEPKTAHIDVHFLKEGCGDDNVCNSNLKLEYKFCTREGNQDKFSYLPIQKGVPELVLKDQKDIALEITVTNSPSNPRNPTKDGDDAHEAKLIATFPDTLTYSAYRELRAFPEKQLSCVANQNGSQADCELGNPFKRNSNVTFYLVLSTTEVTFDTPDLDINLKLETTSNQDNLAPITAKAKVVIELLLSVSGVAKPSQVYFGGTVVGEQAMKSEDEVGSLIEYEFRVINLGKPLTNLGTATLNIQWPKEISNGKWLLYLVKVESKGLEKVTCEPQKEINSLNLTESHNSRKKREITEKQIDDNRKFSLFAERKYQTLNCSVNVNCVNIRCPLRGLDSKASLILRSRLWNSTFLEEYSKLNYLDILMRAFIDVTAAAENIRLPNAGTQVRVTVFPSKTVAQYSGVPWWIILVAILAGILMLALLVFILWKCGFFKRSRYDDSVPRYHAVRIRKEEREIKDEKYIDNLEKKQWITKWNENESYS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P23229 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S13 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| C86 | S-Nitrosylation | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| C131 | S-Nitrosylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| N323 | N-Glycosylation | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| T512 | Phosphorylation | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| C541 | S-Nitrosylation | Uniprot | |
| T560 | Phosphorylation | Uniprot | |
| T588 | Phosphorylation | Uniprot | |
| T592 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| T742 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| Y752 | Phosphorylation | Uniprot | |
| T825 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| Y871 | Phosphorylation | Uniprot | |
| T887 | Phosphorylation | Uniprot | |
| T889 | Phosphorylation | Uniprot | |
| S899 | Phosphorylation | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| T932 | Phosphorylation | Uniprot | |
| S934 | Phosphorylation | Uniprot | |
| S937 | Phosphorylation | Uniprot | |
| K939 | Ubiquitination | Uniprot | |
| S955 | Phosphorylation | Uniprot | |
| K986 | Ubiquitination | Uniprot | |
| N997 | N-Glycosylation | Uniprot | |
| Y1009 | Phosphorylation | Uniprot | |
| S1043 | Phosphorylation | Uniprot | |
| T1045 | Phosphorylation | Uniprot | |
| K1100 | Acetylation | Uniprot | |
| Y1110 | Phosphorylation | Uniprot | |
| K1116 | Ubiquitination | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| Y1129 | Phosphorylation | Uniprot |
Research Backgrounds
Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
Undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner to produce processed integrin alpha-6 (alpha6p). Production of alpha6p enhances prostate cancer cell invasion and migration.
Palmitoylation by DHHC3 enhances stability and cell surface expression.
Cell membrane>Single-pass type I membrane protein. Cell membrane>Lipid-anchor.
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Processed integrin alpha-6: Expressed at low levels in normal prostate tissue with elevated levels in prostate cancer tissue (at protein level).
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond (By similarity). Alpha-6 associates with either beta-1 (ITGB1) or beta-4 (ITGB4) to form ITGA6:ITGB1 and ITGA6:ITGB4, respectively (By similarity). ITGA6:ITGB1 is found in a complex with CD9; interaction takes place in oocytes and is involved in sperm-egg fusion (By similarity). ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3. ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R. ITGA6:ITGB4 interacts with IGF2. Interacts with ADAM9 (By similarity). Interacts with RAB21. Interacts with MDK. ITGA6:ITGB1 interacts with MDK; this interaction mediates MDK-induced neurite outgrowth.
Belongs to the integrin alpha chain family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
References
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