Product: Phospho-Glycogen Synthase (Ser645) Antibody
Catalog: AF3165
Description: Rabbit polyclonal antibody to Phospho-Glycogen Synthase (Ser645)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 83,89kDa; 84kD(Calculated).
Uniprot: P13807
RRID: AB_2834500

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Glycogen Synthase (Ser645) Antibody detects endogenous levels of Glycogen Synthase only when phosphorylated at Serine 645.
RRID:
AB_2834500
Cite Format: Affinity Biosciences Cat# AF3165, RRID:AB_2834500.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Glycogen [starch] synthase; Glycogen synthase 1 (muscle); Glycogen synthase 1; GSY; GYS; Gys1; GYS1_HUMAN; muscle;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
GYS1 muscle glycogen synthase 1. Transfers glucosyl residue from UDP-glucose to glycogen. Regulated allosterically by glucose-6-phosphate, and by PKA-mediated phosphorylation.
Sequence:
MPLNRTLSMSSLPGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNYFLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVYFGRWLIEGGPLVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTWFLGEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRYLCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQITAIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHFYGHLDFNLDKTLYFFIAGRYEFSNKGADVFLEALARLNYLLRVNGSEQTVVAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKFGRKLYESLLVGSLPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
89
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13807 As Substrate

Site PTM Type Enzyme
Phosphorylation
T6 Phosphorylation
S8 Phosphorylation
S10 Phosphorylation
S11 Phosphorylation
Y44 Phosphorylation
K52 Ubiquitination
T75 Phosphorylation
K88 Acetylation
K88 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K100 Acetylation
Y102 Phosphorylation
K231 Ubiquitination
K271 Ubiquitination
K273 Ubiquitination
K285 Ubiquitination
K286 Ubiquitination
K301 Ubiquitination
K381 Ubiquitination
K387 Ubiquitination
K397 Ubiquitination
K403 Ubiquitination
Y405 Phosphorylation
S412 Phosphorylation
K418 Ubiquitination
K422 Ubiquitination
T426 Phosphorylation
K429 Ubiquitination
S484 Phosphorylation
T485 Phosphorylation
S486 Phosphorylation
K599 Ubiquitination
Y604 Phosphorylation
K615 Ubiquitination
Y636 Phosphorylation
S641 Phosphorylation Q96RG2 (PASK) , P49841 (GSK3B) , P49840 (GSK3A) , Q92630 (DYRK2)
S645 Phosphorylation Q15759 (MAPK11) , P49841 (GSK3B)
S647 Phosphorylation
S649 Phosphorylation
S652 Phosphorylation
S653 Phosphorylation P49841 (GSK3B) , Q15759 (MAPK11)
S657 Phosphorylation
Y675 Phosphorylation
K683 Ubiquitination
S698 Phosphorylation
T700 Phosphorylation
S710 Phosphorylation
T713 Phosphorylation
T715 Phosphorylation
S716 Phosphorylation
S717 Phosphorylation
S718 Phosphorylation
T721 Phosphorylation Q15759 (MAPK11)
S723 Phosphorylation
S727 Phosphorylation Q15759 (MAPK11)
T729 Phosphorylation
S730 Phosphorylation
S731 Phosphorylation

Research Backgrounds

Function:

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

PTMs:

Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.

Subunit Structure:

Interacts with GYG1.

Family&Domains:

Belongs to the glycosyltransferase 3 family.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

References

1). Liu HJ et al. A Novel Partial Agonist of Peroxisome Proliferator-Activated Receptor γ with Excellent Effect on Insulin Resistance and Type 2 Diabetes. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 2015 Jun;353(3):573-81 (PubMed: 25876909) [IF=3.5]

Application: WB    Species: human    Sample: HepG2 cells

Fig. 9.| Effect of MDCCCL1636 and pioglitazone on insulin signaling in insulin-resistant cells. HepG2 cells were incubated with or without 100 nM DEX for 24 h and in 1 nM insulin with or without the drugs (pioglitazone or MDCCCL1636)for another 24 h. Cell lysates were separated by SDS-PAGE and subjected to Western blot analysis with (A) anti-phosphoAkt (ser473) antibody, (B)anti-phosphoGSK-3β(Ser9) antibody, and (C) anti-phospho glycogen synthase (Ser645) antibody. Data are expressed as a percentage of the normal control. **p < 0.05, ***p < 0.001 vs. Insulin resistance control; ##p < 0.05, ###p < 0.001 vs. normal control.

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