Product: Phospho-RPA32/RPA2 (Thr21) Antibody
Catalog: AF3183
Description: Rabbit polyclonal antibody to Phospho-RPA32/RPA2 (Thr21)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 35kDa; 29kD(Calculated).
Uniprot: P15927
RRID: AB_2834615

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(88%), Bovine(100%), Sheep(100%), Dog(100%), Xenopus(83%)
Phospho-RPA32/RPA2 (Thr21) Antibody detects endogenous levels of RPA32/RPA2 only when phosphorylated at Threonine 21.
Cite Format: Affinity Biosciences Cat# AF3183, RRID:AB_2834615.
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


60S acidic ribosomal protein P1; AA409079; AI325195; AU020965; ik:tdsubc_2g1; M(2)21C; MGC137236; OTTHUMP00000004008; p32; p34; RCJMB04_6d17 replication protein A2, 32kDa; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; Replication Protein A2 (32kDa); Replication protein A2; Replication protein A2, 32kDa; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; Rpa2; RPA32; RPA34; RpLP1; RpP2; xx:tdsubc_2g1; zgc:109822;


RFA2 is absolutely required for simian virus 40 DNA replication in vitro. It participates in a very early step in initiation. A single-stranded DNA-binding protein.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15927 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S4 Phosphorylation P78527 (PRKDC) , Q8N2W9 (PIAS4)
S8 Phosphorylation Q8N2W9 (PIAS4) , P78527 (PRKDC)
Y9 Phosphorylation
S11 Phosphorylation
S12 Phosphorylation P78527 (PRKDC)
S13 Phosphorylation
Y14 Phosphorylation
T21 Phosphorylation P78527 (PRKDC) , Q13315 (ATM) , Q13535 (ATR)
S23 Phosphorylation Q00535 (CDK5) , P24941 (CDK2) , Q13535 (ATR) , P06493 (CDK1) , P78527 (PRKDC) , Q13315 (ATM)
S29 Phosphorylation Q13315 (ATM) , Q00535 (CDK5) , Q13535 (ATR) , P78527 (PRKDC) , P06493 (CDK1) , P24941 (CDK2)
S33 Phosphorylation P78527 (PRKDC) , Q13535 (ATR) , P06493 (CDK1) , P24941 (CDK2) , Q00535 (CDK5)
K37 Ubiquitination
K38 Ubiquitination
S39 Phosphorylation
S52 Phosphorylation Q13535 (ATR)
S72 Phosphorylation Q13535 (ATR)
K85 Ubiquitination
T88 Phosphorylation
K93 Ubiquitination
R105 Methylation
K127 Ubiquitination
R133 Methylation
K139 Ubiquitination
K171 Ubiquitination
S174 Phosphorylation Q13535 (ATR)
K231 Acetylation
K231 Ubiquitination
K235 Ubiquitination
S238 Phosphorylation
S240 Phosphorylation
K243 Ubiquitination

Research Backgrounds


As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.


Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.

DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination.

Subcellular Location:

Nucleus. Nucleus>PML body.
Note: Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the replication protein A complex (RPA/RP-A), a heterotrimeric complex composed of RPA1, RPA2 and RPA3. Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA). Interacts with SERTAD3. Interacts with TIPIN. Interacts with TIMELESS. Interacts with PPP4R2; the interaction is direct, DNA damage-dependent and mediates the recruitment of the PP4 catalytic subunit PPP4C. Interacts (hyperphosphorylated) with RAD51. Interacts with SMARCAL1; the interaction is direct and mediates the recruitment to the RPA complex of SMARCAL1. Interacts with RAD52 and XPA; those interactions are direct and associate RAD52 and XPA to the RPA complex. Interacts with FBH1. Interacts with ETAA1; the interaction is direct and promotes ETAA1 recruitment at stalled replication forks. Interacts with RFWD3. Interacts with DDI2.


Belongs to the replication factor A protein 2 family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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