Rsk-4 Antibody | Affinity Biosciences

WB
IF/ICC

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Rsk-4 Antibody - Western blot analysis of Rsk-4 using K562 whole cell lysates.

Rsk-4 Antibody - AF9188 staining HepG2 cells by IF/ICC.

Product:	Rsk-4 Antibody
Catalog:	AF9188
Description:	Rabbit polyclonal antibody to Rsk-4
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Rabbit
Mol.Wt.:	84kDa; 84kD(Calculated).
Uniprot:	Q9UK32
RRID:	AB_2843378

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(90%), Horse(80%), Rabbit(80%)

Clonality:

Polyclonal

Specificity:

Rsk-4 Antibody detects endogenous levels of total Rsk-4.

RRID:

AB_2843378
Cite Format: Affinity Biosciences Cat# AF9188, RRID:AB_2843378.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

90 kDa ribosomal protein S6 kinase 6; KS6A6_HUMAN; p90 RSK 6; p90-RSK 6; p90RSK6; pp90RSK4; Ribosomal protein S6 kinase 90kDa polypeptide 6; Ribosomal protein S6 kinase alpha 6; Ribosomal protein S6 kinase alpha-6; Ribosomal S6 kinase 4; rps6ka6; RSK 4; RSK-4; S6K-alpha-6;

Immunogens

Immunogen:

A synthesized peptide derived from human Rsk-4, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9UK32(KS6A6_HUMAN) >>Visit HPA database.

Gene(ID):

RPS6KA6(27330)

Sequence:

Q9UK32 KS6A6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPFAPQDEPWDREMEVFSGGGASSGEVNGLKMVDEPMEEGEADSCHDEGVVKEIPITHHVKEGYEKADPAQFELLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLKKASLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVLFTEEDVKFYLAELALALDHLHQLGIVYRDLKPENILLDEIGHIKLTDFGLSKESVDQEKKAYSFCGTVEYMAPEVVNRRGHSQSADWWSYGVLMFEMLTGTLPFQGKDRNETMNMILKAKLGMPQFLSAEAQSLLRMLFKRNPANRLGSEGVEEIKRHLFFANIDWDKLYKREVQPPFKPASGKPDDTFCFDPEFTAKTPKDSPGLPASANAHQLFKGFSFVATSIAEEYKITPITSANVLPIVQINGNAAQFGEVYELKEDIGVGSYSVCKRCIHATTNMEFAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDVFDDGRYVYLVTDLMKGGELLDRILKQKCFSEREASDILYVISKTVDYLHCQGVVHRDLKPSNILYMDESASADSIRICDFGFAKQLRGENGLLLTPCYTANFVAPEVLMQQGYDAACDIWSLGVLFYTMLAGYTPFANGPNDTPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSWITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKRTSTGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Rabbit

Bovine

Sheep

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9UK32_KS6A6_HUMAN

Function:

Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.

PTMs:

Phosphorylated at Ser-232, Ser-372, and Ser-389 in serum-starved cells.

Subcellular Location:

Cytoplasm>Cytosol. Nucleus.
Note: Predominantly cytosolic.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Rsk-4 Antibody - #AF9188