Product: Phospho-Paxillin (Tyr118) Antibody
Catalog: AF3331
Description: Rabbit polyclonal antibody to Phospho-Paxillin (Tyr118)
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 68kDa; 65kD(Calculated).
Uniprot: P49023
RRID: AB_2834746

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of Paxillin only when phosphorylated at Tyrosine 118.
RRID:
AB_2834746
Cite Format: Affinity Biosciences Cat# AF3331, RRID:AB_2834746.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ16691; FLJ23042; Paired box protein Pax 1; PAX 1; PAX1; PAXI_HUMAN; Paxillin alpha; Paxillin; PXN; PXN protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
PXN is a multi-domain cytoskeletal protein that localizes primarily to focal adhesion sites to the extracellular matrix. Phosphorylated by focal adhesion kinase (FAK) and is a component of integrin signaling. Its phosphorylation provides docking sites for recruitment of signaling molecules to focal adhesions.
Sequence:
MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
91
Sheep
91
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49023 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T29 Phosphorylation
Y31 Phosphorylation A0A059VC25 (FAK) , Q13882 (PTK6) , P00519 (ABL1) , Q05397 (PTK2)
S32 Phosphorylation
Y33 Phosphorylation
T35 Phosphorylation
T39 Phosphorylation
Y40 Phosphorylation P12931 (SRC)
S83 Phosphorylation
S84 Phosphorylation
S85 Phosphorylation Q16539 (MAPK14) , Q00535 (CDK5)
Y88 Phosphorylation P12931 (SRC)
S90 Phosphorylation
S95 Phosphorylation
S103 Phosphorylation
S106 Phosphorylation
C108 S-Nitrosylation
S109 Phosphorylation
Y118 Phosphorylation P00519 (ABL1) , Q05397 (PTK2) , Q13882 (PTK6) , A0A059VC25 (FAK)
S119 Phosphorylation
K123 Ubiquitination
K125 Ubiquitination
S126 Phosphorylation Q9UBE8 (NLK)
S130 Phosphorylation P27361 (MAPK3)
T132 Phosphorylation
S135 Phosphorylation
T136 Phosphorylation
S137 Phosphorylation
S140 Phosphorylation
S143 Phosphorylation
S178 Phosphorylation P45983 (MAPK8)
Y181 Phosphorylation
S188 Phosphorylation
S219 Phosphorylation
S230 Phosphorylation
S243 Phosphorylation
S244 Phosphorylation
T249 Phosphorylation
S250 Phosphorylation
T251 Phosphorylation
T255 Phosphorylation
S258 Phosphorylation Q13153 (PAK1)
S260 Phosphorylation
S261 Phosphorylation
T263 Phosphorylation
S272 Phosphorylation Q13177 (PAK2) , O96013 (PAK4) , Q13153 (PAK1)
S274 Phosphorylation Q13177 (PAK2)
K277 Ubiquitination
S302 Phosphorylation
S303 Phosphorylation
T318 Phosphorylation
S320 Phosphorylation
S321 Phosphorylation
S322 Phosphorylation
S332 Phosphorylation
S336 Phosphorylation
S340 Phosphorylation
K355 Ubiquitination
T371 Phosphorylation
Y409 Phosphorylation
S414 Phosphorylation
Y468 Phosphorylation
S533 Phosphorylation
K540 Ubiquitination

Research Backgrounds

Function:

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

PTMs:

Phosphorylated by MAPK1/ERK2 (By similarity). Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion. Cytoplasm>Cell cortex.
Note: Colocalizes with integrins at the cell periphery. Colocalize with PXN to membrane ruffles and the leading edge of migrating cells (PubMed:23128389).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds in vitro to vinculin as well as to the SH3 domain of SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to PTK2/FAK1 but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to PTK2/FAK1. Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Interacts with PTK2/FAK1 and PTK2B/PYK2. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5 and PDCD10. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with PTK6. Interacts with SORBS1, PARVA and PARVB. Interacts (via cytoplasmic domain) with CEACAM1; the interaction is phosphotyrosyl-dependent. Interacts with LIMA1; this complex stabilizes actin dynamics.

Family&Domains:

Belongs to the paxillin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). Bo H et al. Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro. JOURNAL OF ETHNOPHARMACOLOGY 2018 Nov 14;231:90-97 (PubMed: 30445110) [IF=5.4]

Application: WB    Species: mouse    Sample: BMSCs

Figure 8 |Western blot analysis of key proteins in the focal adhesion pathway after DBT treatment for 6 days. (A, C) Representatives blot from 3 independent experiments. (B, D) Densitometric quantification. GAPDH was used as an internal control. Values are the mean ± SEM, where n = 3. (*p < 0.05 as compared to the control, LSD).

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