Product: Phospho-PKC zeta (Thr410) Antibody
Catalog: AF3404
Description: Rabbit polyclonal antibody to Phospho-PKC zeta (Thr410)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70kDa; 68kD(Calculated).
Uniprot: Q05513
RRID: AB_2834787

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 100ul $280 In stock
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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Phospho-PKC zeta (Thr410) Antibody detects endogenous levels of PKC zeta only when phosphorylated at Threonine 410.
Cite Format: Affinity Biosciences Cat# AF3404, RRID:AB_2834787.
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


14-3-3-zetaisoform; AI098070; aPKCzeta; C80388; EC; KPCZ_HUMAN; nPKC zeta; nPKC-zeta; OTTHUMP00000001368; OTTHUMP00000044160; PKC 2; PKC ZETA; PKC2; Pkcz; PKCZETA; PKM-zeta, included; PRKCZ; Protein kinase C zeta; Protein kinase C zeta form; Protein kinase C zeta type; r14-3-3; R74924; zetaPKC;



Expressed in brain, and to a lesser extent in lung, kidney and testis.

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05513 As Substrate

Site PTM Type Enzyme
S148 Phosphorylation
S190 Phosphorylation
S206 Phosphorylation
S223 Phosphorylation
S228 Phosphorylation
T407 Phosphorylation
S409 Phosphorylation
T410 Phosphorylation O15530 (PDPK1)
Y417 Phosphorylation
Y428 Phosphorylation
T560 Phosphorylation Q05513 (PRKCZ)

PTMs - Q05513 As Enzyme

Substrate Site Source
O14920 (IKBKB) S177 Uniprot
O14920 (IKBKB) S181 Uniprot
O14965 (AURKA) T287 Uniprot
O43175 (PHGDH) S55 Uniprot
O43175 (PHGDH) T57 Uniprot
O43175 (PHGDH) T78 Uniprot
O43315 (AQP9) S11 Uniprot
O43315 (AQP9) S222 Uniprot
P05023 (ATP1A1) S16 Uniprot
P08047 (SP1) S641 Uniprot
P08047 (SP1) T668 Uniprot
P08047 (SP1) S670 Uniprot
P08047 (SP1) T681 Uniprot
P10636-8 (MAPT) S258 Uniprot
P10636-8 (MAPT) S293 Uniprot
P10636-8 (MAPT) S324 Uniprot
P10636-8 (MAPT) S352 Uniprot
P14598 (NCF1) S303 Uniprot
P14598 (NCF1) S304 Uniprot
P14598 (NCF1) S315 Uniprot
P14598 (NCF1) S320 Uniprot
P14598 (NCF1) S328 Uniprot
P14598 (NCF1) S359 Uniprot
P14598 (NCF1) S370 Uniprot
P14598 (NCF1) S379 Uniprot
P17252 (PRKCA) S657 Uniprot
P27448 (MARK3) T564 Uniprot
P28329-3 (CHAT) S440 Uniprot
P28329-3 (CHAT) S476 Uniprot
P28329 (CHAT) S558 Uniprot
P28329 (CHAT) S594 Uniprot
P29474 (NOS3) T495 Uniprot
P30086 (PEBP1) S153 Uniprot
P31749 (AKT1) T34 Uniprot
P31946-1 (YWHAB) S132 Uniprot
P31946-1 (YWHAB) T143 Uniprot
P35222 (CTNNB1) S45 Uniprot
P35568 (IRS1) S323 Uniprot
P35568 (IRS1) S441 Uniprot
P35568 (IRS1) S1101 Uniprot
P35580 (MYH10) S1937 Uniprot
P35611 (ADD1) S726 Uniprot
P35612 (ADD2) S713 Uniprot
P41594-2 (GRM5) S840 Uniprot
P46937 (YAP1) S109 Uniprot
P46937 (YAP1) T110 Uniprot
P49757-2 (NUMB) S7 Uniprot
P49757-2 (NUMB) S295 Uniprot
P49768 (PSEN1) S346 Uniprot
P49841 (GSK3B) S9 Uniprot
P49841 (GSK3B) S147 Uniprot
P55040 (GEM) S261 Uniprot
P55211 (CASP9) S144 Uniprot
P60484 (PTEN) S380 Uniprot
P60484 (PTEN) T382 Uniprot
P60484 (PTEN) T383 Uniprot
P61587 (RND3) S210 Uniprot
Q04206 (RELA) S311 Uniprot
Q05513-2 (PRKCZ) T377 Uniprot
Q05513 (PRKCZ) T560 Uniprot
Q05655-1 (PRKCD) S664 Uniprot
Q07812 (BAX) S184 Uniprot
Q12933 (TRAF2) S55 Uniprot
Q13164 (MAPK7) S486 Uniprot
Q13469 (NFATC2) S53 Uniprot
Q13469 (NFATC2) S56 Uniprot
Q15139 (PRKD1) S738 Uniprot
Q15139 (PRKD1) S742 Uniprot
Q15466 (NR0B2) T55 Uniprot
Q15831 (STK11) S307 Uniprot
Q15831-2 (STK11) S399 Uniprot
Q15831 (STK11) S428 Uniprot
Q16612 (NREP) S59 Uniprot
Q16625 (OCLN) T403 Uniprot
Q16625 (OCLN) T404 Uniprot
Q16625 (OCLN) T424 Uniprot
Q16625 (OCLN) T438 Uniprot
Q5JVS0-1 (HABP4) T354 Uniprot
Q5JVS0 (HABP4) T375 Uniprot
Q6STE5 (SMARCD3) S247 Uniprot
Q7KZI7 (MARK2) T596 Uniprot
Q8IX03-1 (WWC1) S975 Uniprot
Q8IX03-1 (WWC1) S978 Uniprot
Q8TEW0 (PARD3) S827 Uniprot
Q96A00-1 (PPP1R14A) T38 Uniprot
Q96RI1-2 (NR1H4) T442 Uniprot
Q96RI1 (NR1H4) T456 Uniprot
Q9UBS0 (RPS6KB2) S473 Uniprot
Q9Y243 (AKT3) T305 Uniprot
Q9Y243 (AKT3) S472 Uniprot
Q9Y6Q9 (NCOA3) S1033 Uniprot
Q9Y6Q9 (NCOA3) S1042 Uniprot
Q9Y6Q9 (NCOA3) S1048 Uniprot
Q9Y6Q9 (NCOA3) T1059 Uniprot
Q9Y6Q9 (NCOA3) S1062 Uniprot
Q9Y6Q9 (NCOA3) T1064 Uniprot
Q9Y6Q9 (NCOA3) T1067 Uniprot
Q9Y6Q9 (NCOA3) T1114 Uniprot

Research Backgrounds


Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis. Phosphorylates VAMP2 in vitro.

Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.


CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase.

Subcellular Location:

Cytoplasm. Endosome. Cell junction. Membrane>Peripheral membrane protein.
Note: In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes (PubMed:9566925). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083). Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651). Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity).


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in brain, and to a lesser extent in lung, kidney and testis.

Subunit Structure:

Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 (By similarity). Interacts with PARD6A, PARD6B, PARD6G and SQSTM1. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Forms a ternary complex composed of SQSTM1 and PAWR. Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminal region). Interacts with WDFY2 (via WD repeats 1-3). Interacts with VAMP2. Forms a complex with WDFY2 and VAMP2. Interacts with APPL1.


The PB1 domain mediate mutually exclusive interactions with SQSTM1 and PARD6B.

The C1 domain does not bind the diacylglycerol (DAG).

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

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