Phospho-PYGL (Ser15) Antibody | Affinity Biosciences

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Product:	Phospho-PYGL (Ser15) Antibody
Catalog:	AF3863
Description:	Rabbit polyclonal antibody to Phospho-PYGL (Ser15)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	102kDa; 97kD(Calculated).
Uniprot:	P06737
RRID:	AB_2847177

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-PYGL (Ser15) Antibody detects endogenous levels of PYGL only when phosphorylated at Ser15.

RRID:

AB_2847177
Cite Format: Affinity Biosciences Cat# AF3863, RRID:AB_2847177.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glycogen phosphorylase; Glycogen phosphorylase L; Glycogen phosphorylase liver; Glycogen phosphorylase liver form; GSD6; Hers disease, glycogen storage disease type VI; liver form; OTTHUMP00000233649; OTTHUMP00000233651; Phosphorylase glycogen liver; Pygl; PYGL_HUMAN;

Immunogens

Immunogen:

Uniprot:

P06737(PYGL_HUMAN) >>Visit HPA database.

Gene(ID):

PYGL(5836)

Sequence:

P06737 PYGL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

PTMs - P06737 As Substrate

P06737

Site	PTM Type	Source
A2	Acetylation	Uniprot
K3	Ubiquitination	Uniprot
T6	Phosphorylation	Uniprot
K10	Ubiquitination	Uniprot
S15	Phosphorylation	Uniprot
K29	Acetylation	Uniprot
K29	Ubiquitination	Uniprot
K30	Ubiquitination	Uniprot
S31	Phosphorylation	Uniprot
T39	Phosphorylation	Uniprot
K42	Ubiquitination	Uniprot
Y75	Phosphorylation	Uniprot
Y76	Phosphorylation	Uniprot
R82	Methylation	Uniprot
Y85	Phosphorylation	Uniprot
Y114	Phosphorylation	Uniprot
K170	Ubiquitination	Uniprot
Y186	Phosphorylation	Uniprot
K192	Ubiquitination	Uniprot
S193	Phosphorylation	Uniprot
Y204	Phosphorylation	Uniprot
K206	Ubiquitination	Uniprot
R248	Methylation	Uniprot
Y263	Phosphorylation	Uniprot
Y281	Phosphorylation	Uniprot
K290	Ubiquitination	Uniprot
K295	Ubiquitination	Uniprot
K316	Ubiquitination	Uniprot
K359	Ubiquitination	Uniprot
S363	Phosphorylation	Uniprot
K364	Ubiquitination	Uniprot
K371	Ubiquitination	Uniprot
K395	Ubiquitination	Uniprot
Y405	Phosphorylation	Uniprot
K410	Ubiquitination	Uniprot
R414	Methylation	Uniprot
K421	Ubiquitination	Uniprot
S430	Phosphorylation	Uniprot
K438	Ubiquitination	Uniprot
S450	Phosphorylation	Uniprot
K465	Ubiquitination	Uniprot
K470	Acetylation	Uniprot
K470	Ubiquitination	Uniprot
S473	Phosphorylation	Uniprot
K479	Ubiquitination	Uniprot
K483	Ubiquitination	Uniprot
K514	Ubiquitination	Uniprot
S517	Phosphorylation	Uniprot
K521	Ubiquitination	Uniprot
S524	Phosphorylation	Uniprot
K545	Ubiquitination	Uniprot
S547	Phosphorylation	Uniprot
K555	Ubiquitination	Uniprot
K557	Ubiquitination	Uniprot
K569	Ubiquitination	Uniprot
K575	Acetylation	Uniprot
K609	Ubiquitination	Uniprot
S626	Phosphorylation	Uniprot
S639	Phosphorylation	Uniprot
K640	Ubiquitination	Uniprot
K642	Ubiquitination	Uniprot
K656	Ubiquitination	Uniprot
K724	Acetylation	Uniprot
K724	Ubiquitination	Uniprot
K725	Ubiquitination	Uniprot
K730	Ubiquitination	Uniprot
S752	Phosphorylation	Uniprot
K754	Ubiquitination	Uniprot
Y778	Phosphorylation	Uniprot
K783	Ubiquitination	Uniprot
K787	Ubiquitination	Uniprot
K796	Ubiquitination	Uniprot
K804	Ubiquitination	Uniprot
K811	Ubiquitination	Uniprot
R816	Methylation	Uniprot
K819	Ubiquitination	Uniprot
K834	Acetylation	Uniprot
K834	Ubiquitination	Uniprot
S836	Phosphorylation	Uniprot
S838	Phosphorylation	Uniprot
K843	Ubiquitination	Uniprot

Research Backgrounds

P06737_PYGL_HUMAN

Function:

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

PTMs:

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Subunit Structure:

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Interacts with PPP1R3B (By similarity).

Family&Domains:

Belongs to the glycogen phosphorylase family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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For Research Use Only.
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Phospho-PYGL (Ser15) Antibody - #AF3863