Product: SREBP1 Antibody
Catalog: AF6283
Description: Rabbit polyclonal antibody to SREBP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Pig, Sheep
Prediction: Pig, Horse, Sheep, Dog
Mol.Wt.: 122kD, 65kD(cleaved); 122kD(Calculated).
Uniprot: P36956
RRID: AB_2835134

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Pig,Sheep
Prediction:
Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SREBP1 Antibody detects endogenous levels of total SREBP1.
RRID:
AB_2835134
Cite Format: Affinity Biosciences Cat# AF6283, RRID:AB_2835134.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P36956 SRBP1_HUMAN:

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Description:
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum.
Sequence:
MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPGSLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPLPGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVPQQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGPLPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLVVGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTEVEDTLTPPPSDAGSPFQSSPLSLGSRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLALCTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVSLVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSALNLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHRFFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYLQLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQESERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVVRTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLMRLGGGTTVTSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36956 As Substrate

Site PTM Type Enzyme
T59 Phosphorylation
S63 Phosphorylation Q16539 (MAPK14)
S117 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P45983 (MAPK8)
K123 Sumoylation
K256 Ubiquitination
T275 Phosphorylation
S312 Phosphorylation
K313 Ubiquitination
R321 Methylation
K324 Acetylation
K324 Ubiquitination
K333 Acetylation
S337 Phosphorylation P57059 (SIK1)
S338 Phosphorylation P57059 (SIK1) , P17612 (PRKACA)
K342 Ubiquitination
K347 Ubiquitination
K356 Ubiquitination
K359 Ubiquitination
S360 Phosphorylation
K365 Ubiquitination
K379 Ubiquitination
K381 Ubiquitination
S396 Phosphorylation P54646 (PRKAA2)
K398 Ubiquitination
S402 Phosphorylation P57059 (SIK1)
K418 Sumoylation
T424 Phosphorylation
T426 Phosphorylation P49841 (GSK3B) , Q16539 (MAPK14)
S430 Phosphorylation P49841 (GSK3B)
S434 Phosphorylation P49841 (GSK3B)
S439 Phosphorylation P06493 (CDK1)
S448 Phosphorylation
S450 Phosphorylation
S455 Phosphorylation
S457 Phosphorylation
S467 Phosphorylation P53350 (PLK1)
K470 Ubiquitination
S486 Phosphorylation
Y567 Phosphorylation
K587 Ubiquitination
K675 Ubiquitination
K727 Ubiquitination
K924 Methylation
K924 Ubiquitination
K934 Ubiquitination
K947 Ubiquitination
S1020 Phosphorylation
S1027 Phosphorylation
S1049 Phosphorylation
S1060 Phosphorylation
K1070 Ubiquitination
K1121 Ubiquitination

Research Backgrounds

Function:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').

PTMs:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Nucleus.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.

Subunit Structure:

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Interacts with CEBPA, the interaction produces a transcriptional synergy (By similarity).

Family&Domains:

The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Belongs to the SREBP family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

References

1). Wang Y et al. Overexpression of NAG-1/GDF15 prevents hepatic steatosis through inhibiting oxidative stress-mediated dsDNA release and AIM2 inflammasome activation. Redox Biology 2022 Jun;52:102322. (PubMed: 35504134) [IF=11.4]

2). Yang Y et al. Amelioration of nonalcoholic fatty liver disease by swertiamarin in fructose-fed mice. Phytomedicine 2019 Jun;59:152782 (PubMed: 31005808) [IF=7.9]

Application: IHC    Species: mouse    Sample: liver

Figure.7. |Effect of swertiamarin (25, 50 and 100 mg/kg) and silibinin on (A) ACC1 expression by western blotting analysis. Immunohistochemical stainings of (B) SREBP-1 and (C) FAS in liver tissue of mice. Scale bar represents 30 μm. Representative analysis from six animals in each group.Significance is represented as ##P ≤ 0.01 and ###P ≤ 0.001 compared to the normal control group,*P ≤ 0.05 and **P ≤ 0.01 compared to the fructose group.

3). Xu N et al. β-patchoulene improves lipid metabolism to alleviate non-alcoholic fatty liver disease via activating AMPK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY 2021 Feb;134:111104. (PubMed: 33341045) [IF=7.5]

Application: WB    Species: Human    Sample: L02 cell

Fig. 4. β-PAE inhibits the expression of hepatic lipid synthesis-related proteins and genes in HFD-fed rats. (A–F) Western blot analysis on the expression of proteins referred to hepatic lipid synthesis including SREBP-1c, ACC1, p-ACC1, FASN, SCD1 and HMG-CR; (G–H) The mRNA expression of SREBP-1c and HMG-CR. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.

4). Luan H et al. Scutellarin, a modulator of mTOR, attenuates hepatic insulin resistance by regulating hepatocyte lipid metabolism via SREBP‐1c suppression. PHYTOTHERAPY RESEARCH 2020 Jun;34(6):1455-1466. (PubMed: 31828866) [IF=7.2]

Application: WB    Species: Human    Sample: HepG2 cells

FIGURE 2 Effects of Scu on the mTOR/SREBP-1c signalling pathway in HepG2 cells induced by PA. (a) and (g) Representative Western blot images with insulin stimulation. (b) The phosphorylation of mTOR. (c) The level of SREBP-1c protein expression. (d) Representative Western blot images without insulin stimulation. (e) The phosphorylation of mTOR. (f) The level of SREBP-1c protein expression. (h) The level of n-SREBP-1c protein expression. Data are expressed as the mean ± SEM (n = 3). ##p < .01 versus the insulin only treated group or untreated group; *p < .05, **p < .01 versus the control (treated with PA only or PA plus insulin) group. mTOR, mammalian target of rapamycin; PA, palmitic acid; Scu, Scutellarin

5). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.

6). Xu Q et al. Propionate Ameliorates Alcohol-Induced Liver Injury in Mice via the Gut–Liver Axis: Focus on the Improvement of Intestinal Permeability. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022 May 12. (PubMed: 35549256) [IF=6.1]

7). Tang C et al. Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2023 Jan 25;71(3):1464-1476. (PubMed: 36695046) [IF=6.1]

8). Luo H et al. β-patchoulene protects against non-alcoholic steatohepatitis via interrupting the vicious circle among oxidative stress, histanoxia and lipid accumulation in rats. International Immunopharmacology 2021 Sep;98:107915. (PubMed: 34198236) [IF=5.6]

Application: WB    Species: Rat    Sample:

Fig. 8. Effect of β-PAE on AMPK signalling pathway. (A) The hepatic mRNA expressions of AMPKα, SREBP-1c and PPARα. (B) Representative bands of p-AMPKα, AMPKα, SREBP-1c and PPARα. (C) Quantitative results of Western blot bands densities of p-AMPKα/ AMPKα, SREBP-1c and PPARα. (D) Spearman correlation between hepatic CD36 expression and AMPK signalling pathway-related proteins indicators, respectively. Data are presented as the mean ± SD (n = 3 ~ 6). ##P < 0.01 vs. NC group; **P < 0.01 vs. Model group.

9). Protective effects of Lactobacillus acidophilus NX2-6 against oleic acid-induced steatosis, mitochondrial dysfunction, endoplasmic reticulum stress and inflammatory responses. Journal of Functional Foods [IF=5.6]

10). Xu L et al. Patchouli alcohol ameliorates acute liver injury via inhibiting oxidative stress and gut-origin LPS leakage in rats. International Immunopharmacology 2021 Jun 25;98:107897. (PubMed: 34182243) [IF=5.6]

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