PI4KB Antibody - #DF15254

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Product: PI4KB Antibody
Catalog: DF15254
Description: Rabbit polyclonal antibody to PI4KB
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 91kD(Calculated).
Uniprot: Q9UBF8

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
PI4KB Antibody detects endogenous levels of PI4KB.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from human PI4KB.

Uniprot:

Q9UBF8(PI4KB_HUMAN) >>Visit HPA database.

Gene(ID):
PI4KB(5298)
Expression:
Q9UBF8 PI4KB_HUMAN:

Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.

Sequence:
MGDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM

PTMs - Q9UBF8 As Substrate

Site PTM Type Enzyme
G2 Acetylation
K60 Ubiquitination
Y147 Phosphorylation
Y175 Phosphorylation
Y183 Phosphorylation
S205 Phosphorylation
S209 Phosphorylation
S256 Phosphorylation
S258 Phosphorylation
T263 Phosphorylation
S266 Phosphorylation P06493 (CDK1)
S268 Phosphorylation
S275 Phosphorylation
S277 Phosphorylation Q15208 (STK38)
T280 Phosphorylation
S286 Phosphorylation
S287 Phosphorylation
K290 Methylation
T292 Phosphorylation
S294 Phosphorylation Q15139 (PRKD1) , Q9BZL6 (PRKD2)
S306 Phosphorylation
K337 Ubiquitination
T341 Phosphorylation
K345 Ubiquitination
T349 Phosphorylation
K362 Ubiquitination
K392 Ubiquitination
S425 Phosphorylation
T426 Phosphorylation
S428 Phosphorylation
T438 Phosphorylation
S511 Phosphorylation
T517 Phosphorylation
T519 Phosphorylation
K534 Ubiquitination
K596 Ubiquitination
K742 Acetylation
Y805 Phosphorylation
Y810 Phosphorylation

Research Backgrounds

Function:

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).

(Microbial infection) Plays an essential role in Aichi virus RNA replication. Recruited by ACBD3 at the viral replication sites.

Subcellular Location:

Endomembrane system. Mitochondrion outer membrane>Peripheral membrane protein. Rough endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus. Golgi apparatus membrane. Cytoplasm>Perinuclear region.
Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex. Recruited to the Golgi apparatus membrane by ACBD3 (PubMed:24672044, PubMed:27009356, PubMed:28289207). GGA2 is also involved in the recruitment (PubMed:28289207).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.

Subunit Structure:

Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6. Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3. Interacts with ARMH3, YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN. Interacts with GGA2 (via VHS domain); the interaction is important for PI4KB location at the Golgi apparatus membrane.

(Microbial infection) Interacts with Aichi virus protein 3A. Part of a complex Aichi virus protein 3A/ACBD3/PI4KB that allows the synthesis of PI4P at the viral RNA replication sites.

Family&Domains:

Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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