Carbonic anhydrase 2/CA2 Recombinant Rabbit mAb - #BF4295

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Product: Carbonic anhydrase 2/CA2 Recombinant Rabbit mAb
Catalog: BF4295
Description: Rabbit monoclonal antibody to Carbonic anhydrase 2/CA2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 29 kDa(Observed); 29kD(Calculated).
Uniprot: P00918

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:200-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23745]
Specificity:
Carbonic anhydrase 2/CA2 Recombinant Rabbit mAb detects endogenous levels of Carbonic anhydrase 2/CA2.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CA 2; CA II; CA-II; Ca2; CAC; CAH2_HUMAN; CAII; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase 2; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase C, formerly; Carbonic anhydrase II; Carbonic dehydratase; epididymis luminal protein 76; Epididymis secretory protein Li 282; HEL-76; HEL-S-282;

Immunogens

Immunogen:

A synthetic peptide from human Carbonic anhydrase 2

Uniprot:

P00918(CAH2_HUMAN) >>Visit HPA database.

Gene(ID):
CA2(760)
Description:
The protein encoded by this gene is one of several isozymes of carbonic anhydrase,which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.
Sequence:
MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

Research Backgrounds

Function:

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the alpha-carbonic anhydrase family.

Research Fields

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Excretory system > Collecting duct acid secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

Restrictive clause

 

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For Research Use Only.
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