Angiopoietin 2 Antibody - #AF5124

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Product: Angiopoietin 2 Antibody
Catalog: AF5124
Description: Rabbit polyclonal antibody to Angiopoietin 2
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 55~70kDa; 57kD(Calculated).
Uniprot: O15123
RRID: AB_2837610

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%)
Clonality:
Polyclonal
Specificity:
Angiopoietin 2 Antibody detects endogenous levels of total Angiopoietin 2.
RRID:
AB_2837610
Cite Format: Affinity Biosciences Cat# AF5124, RRID:AB_2837610.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGPT 2; Agpt2; ANG 2; ANG-2; ANG2; Angiopoietin 2a; Angiopoietin 2B; Angiopoietin-2; Angiopoietin2; ANGP2_HUMAN; ANGPT 2; Angpt2; Tie2 ligand;

Immunogens

Immunogen:

A synthesized peptide derived from human Angiopoietin 2, corresponding to a region within N-terminal amino acids.

Uniprot:

O15123(ANGP2_HUMAN) >>Visit HPA database.

Gene(ID):
ANGPT2(285)
Description:
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression.
Sequence:
MWQIVFFTLSCDLVLAAAYNNFRKSMDSIGKKQYQVQHGSCSYTFLLPEMDNCRSSSSPYVSNAVQRDAPLEYDDSVQRLQVLENIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLAMEDKHIIQLQSIKEEKDQLQVLVSKQNSIIEELEKKIVTATVNNSVLQKQQHDLMETVNNLLTMMSTSNSAKDPTVAKEEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
78
Dog
78
Rabbit
78
Bovine
53
Sheep
53
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

References

1). hUCMSCs restore ovarian function via angiopoietin 1/2 rebalance in POI rats. Journal of ovarian research, 2025 (PubMed: 41254793) [IF=4.0]

Application: IF/ICC    Species: human    Sample:

Fig. 4 hUCMSCs restore ovarian angiogenesis by rebalancing Angpt1/Angpt2 in POI. A Immunofluorescence detection of Angpt1, Angpt2 and CD31 expression in the ovaries from control, POI and POI + hUCMSCs group. DAPI repainted nucleus are blue, Angpt1 and Angpt2 positive are green, CD31 positive is red. scale bar is 60 μm. B Western blot of CD31, Angpt1, Angpt2, and GAPDH in ovarian tissues across groups. C-E Quantitative analysis of Angpt1, Angpt2 and CD31(normalized to GAPDH). F. The ratio of Angpt1/Angpt2 at protein level. G-I Quantification of Angpt1, Angpt2 and mRNA expression ratio. J. Correlation between Angpt1/Angpt2 protein ratio and CD31 at protein level. Data were shown as mean ± SD of at least three independent experiments. *: P

Application: WB    Species: human    Sample:

Fig. 4 hUCMSCs restore ovarian angiogenesis by rebalancing Angpt1/Angpt2 in POI. A Immunofluorescence detection of Angpt1, Angpt2 and CD31 expression in the ovaries from control, POI and POI + hUCMSCs group. DAPI repainted nucleus are blue, Angpt1 and Angpt2 positive are green, CD31 positive is red. scale bar is 60 μm. B Western blot of CD31, Angpt1, Angpt2, and GAPDH in ovarian tissues across groups. C-E Quantitative analysis of Angpt1, Angpt2 and CD31(normalized to GAPDH). F. The ratio of Angpt1/Angpt2 at protein level. G-I Quantification of Angpt1, Angpt2 and mRNA expression ratio. J. Correlation between Angpt1/Angpt2 protein ratio and CD31 at protein level. Data were shown as mean ± SD of at least three independent experiments. *: P
2). Perillyl alcohol attenuates hypoxia induced right ventricular dysfunction and remodeling by balancing the renin angiotensin aldosterone system in rats. Scientific reports, 2026 (PubMed: 41644575) [IF=3.8]
3). Bone marrow-derived mesenchymal stem cells transplantation attenuates renal fibrosis following acute kidney injury by repairing the peritubular capillaries. Experimental Cell Research, 2022 (PubMed: 34921827) [IF=3.3]
4). Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation. Biology of Reproduction, 2019 (PubMed: 30084973) [IF=3.1]
5). Effect of electroacupuncture on hypertensive and sympathetic excitability mechanism mediated by the paraventricular nucleus of the hypothalamus in spontaneous hypertensive rats. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 2025 (PubMed: 40524297) [IF=2.0]

Application: WB    Species: Rat    Sample:

Figure 2. NMDAR, Ang Ⅱ and AT1protein expression in each group of rats. A: Western blot analysis of levels of NMDAR and Ang Ⅱ expression in PVN of rats in each group; B: comparison of the protein amount of NMDAR in each group; C: comparison of the protein amount of Ang Ⅱ in each group; D: expression of AT1 mRNA. 1: model group; 2: control group; 3: EA group; 4: sham group; 5: NRA + EA group. Control and model groups: fixed with a rat sleeve (as per rats in the EA and NRA + EA groups) without any acupuncture intervention for 14 d; Sham group: inject the Artificial cerebrospinal fluid (10 mmol/L, 100 nL) and fixed with a rat sleeve (as per rats in the EA and NRA + EA groups) without any acupuncture intervention for 14 d; EA group: electro-acupuncture at Taichong (LR3) and Quchi (LI11) acupoints for 14 d; NRA + EA group: inject the N-methyl-D-aspartic acid receptor inhibitor (10 mmol/L, 100 nL) and electro-acupuncture at Taichong (LR3) and Quchi (LI11) acupoints for 14 d. EA: Electroacupuncture; NRA + EA: N-methyl-D-aspartate receptor antagonist and electroacupuncture; NMDAR: N-methyl-D-aspartate receptor; Ang II: angiotensin II; AT1: angiotensin II type 1. One-way analysis of variance was adopted, and the least significant difference t-test was used for the comparison among groups. Data were presented as mean ± standard deviation (n = 16). aP < 0.01 and eP < 0.05, vs control group; bP < 0.05 and fP < 0.01, vs model group; cP < 0.05 and gP < 0.01, vs Sham group; dP < 0.05, vs EA group.
6). Distinct effects of ANGPT2 on gene expression of glomerular podocytes and mesangial cells. American Journal of Translational Research, 2021 (PubMed: 34956451) [IF=1.7]

Application: IF/ICC    Species: human and mouse    Sample: glomerular cells

Figure 2 ANGPT2 protein expression in glomerular cells of human and mouse. A. Immunofluorescence staining of ANGPT2 in normal human glomeruli. Podocyte marker, SYNPO, was co-stained, thereby localizing ANGPT2 to podocytes and non-podocytes. B. Similar co-staining of ANGPT2 and SYNPO in mouse glomeruli showing ANGPT2 is present in both podocytes and non-podocytes in mice.

Application: WB    Species: Human    Sample: mesangial cells

Figure 10 pAKT was increased in ANGPT2-treated podocytes but downregulated in mesangial cells in culture. Podocytes and mesangial cells were treated with 500 ng/ml ANGPT2 for 24 h and subject to immnunoblotting. The results represent the data from three independent experiments and are expressed as mean ± SD. **P<0.01 vs. control.
7). [Dose and timing of normal saline resuscitation on endothelial glycocalyx in early septic shock]. , 2018 (PubMed: 30045788)
8). Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1 α and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma Rat Model. Evidence-based complementary and alternative medicine : eCAM, 2020 (PubMed: 32328124)

Application: WB    Species: Rat    Sample:

Figure 1 (e) In the XYK group, HIF-1α and VEGF decreased, E3 ubiquitin-protein ligase parkin and 26S proteasome protein increased, and the Ang-1/Ang-2 ratio increased in the hematoma. Western blots analysis of HIF-1α, E3 ubiquitin-protein ligase parkin, and 26S proteasome protein in hematoma (A-D). Western blots analysis of VEGF, Ang-1, and Ang-2 (E-I).  ∗P < 0.05 and  ∗∗P < 0.01. Ang, angiopoietins; CSDH, chronic subdural hematoma HIF, hypoxia-inducible factor; VEGF, vascular endothelial growth factor; XYK, Xiaoyukang Jiaonang.

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