HDAC5 Antibody - #AF5348

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Product: HDAC5 Antibody
Catalog: AF5348
Description: Rabbit polyclonal antibody to HDAC5
Application: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 122 kDa; 122kD(Calculated).
Uniprot: Q9UQL6
RRID: AB_2837833

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(93%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HDAC5 Antibody detects endogenous levels of total HDAC5.
RRID:
AB_2837833
Cite Format: Affinity Biosciences Cat# AF5348, RRID:AB_2837833.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen NY CO 9; Antigen NY-CO-9; HD5; HDAC 5; HDAC5; HDAC5_HUMAN; Histone deacetylase 5; NY CO 9;

Immunogens

Immunogen:

A synthesized peptide derived from human HDAC5, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9UQL6(HDAC5_HUMAN) >>Visit HPA database.

Gene(ID):
HDAC5(10014)
Expression:
Q9UQL6 HDAC5_HUMAN:

Ubiquitous.

Description:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Sequence:
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
93
Xenopus
77
Zebrafish
69
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.

PTMs:

Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).

Ubiquitinated. Polyubiquitination however does not lead to its degradation.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Belongs to the histone deacetylase family. HD type 2 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

References

1). Lactate Dehydrogenase Inhibition Protects against Hepatic Fibrosis by Regulating Metabolic Reprogramming of Hepatic Stellate Cells. Journal of agricultural and food chemistry, 2024 (PubMed: 39632278) [IF=6.1]
2). WNT10A induces apoptosis of senescent synovial resident stem cells through Wnt/calcium pathway-mediated HDAC5 phosphorylation in OA joints. BONE, 2021 (PubMed: 34000432) [IF=3.5]

Application: IHC    Species: human    Sample: Sn-SMSCs

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μm) and caspase3 (scale bars = 100 μm) after 0/1/24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A(n = 5).

Application: WB    Species: human    Sample: Sn-SMSCs

Fig. 4. |Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phosphorylation at s498 (right); *P<0.05

Application: IHC    Species: Rat    Sample: senescent cells (SnCs)

Fig. 4. Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: A: RNA-seq results after 3 days of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A (n = 3). A: Top 10 hub proteins (left) and PPI (right). B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μ m) and caspase3 (scale bars = 100 μ m) after 0/1/ 24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A (n = 5). C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phos- phorylation at s498 (right); *P<0.05.

Application: WB    Species: Rat    Sample: senescent cells (SnCs)

Fig. 4. Histone deacetylase 5 (HDAC5) is involved in WNT10A-induced apoptosis of Sn-SMSCs: A: RNA-seq results after 3 days of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A (n = 3). A: Top 10 hub proteins (left) and PPI (right). B: Immunohistochemistry staining for HDAC5 (scale bars = 50 μ m) and caspase3 (scale bars = 100 μ m) after 0/1/ 24/72 h of culture in complete medium supplemented with 50 ng/mL WNT10A (n = 5). C: HDAC5 mRNA (left), protein (middle) levels. and HDAC5 phos- phorylation at s498 (right); *P<0.05.

Application: IF/ICC    Species: Rat    Sample: senescent cells (SnCs)

Fig. 5. WNT10A phosphorylates HDAC5 via the Wnt/calcium pathway: A: Expression of β-catenin and GSK3β (left, upper); expression of p-JNK and JNK (left, lower); expression of p-PKC and PKC, p-CaMKII and CaMKII, p-NFAT, and NFAT (middle); ratio of p-PKC:PKC, p-CaMKII:CaMKII and p-NFAT:NFAT (right) after 24 h of culture in complete medium supplemented with PBS (control) or 50 ng/mL WNT10A. B: Measurement of intracellular Ca2+ concentration using Fluo-4 AM. C: Expression of p-CaMKII and CaMKII, p-HDAC5 s498 and HDAC5, and p53 and acetylation p53 at K120 (upper); ratio of p-CaMKII:CaMKII and p-HDAC5 s498:HDAC5 (lower) after 24 h of culture in complete medium supplemented with PBS (control), 50 ng/mL WNT10A, or 50 ng/mL WNT10A + 10 μ M KN-62 (an inhibitor of CaMKII) (n = 5). D: Immunofluorescence staining for HDAC5 after 24 h of culture in complete medium supplemented with PBS (control), 50 ng/mL WNT10A, or 50 ng/mL WNT10A + 10 μ M KN-62 (n = 5); scale bars = 50 μ m; *P<0.05, **P<0.01.

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