Product: Cytochrome P450 7A1 Antibody
Catalog: DF2612
Description: Rabbit polyclonal antibody to Cytochrome P450 7A1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 58 kDa; 58kD(Calculated).
Uniprot: P22680
RRID: AB_2839818

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Horse(88%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytochrome P450 7A1 Antibody detects endogenous levels of total Cytochrome P450 7A1.
RRID:
AB_2839818
Cite Format: Affinity Biosciences Cat# DF2612, RRID:AB_2839818.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cholesterol 7 alpha hydroxylase; Cholesterol 7 alpha monooxygenase; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; CP7A; CP7A1_HUMAN; CYP 7; CYP7; CYP7A1; CYPVII; Cytochrome P450 7A1; Cytochrome P450, family 7, subfamily A, polypeptide 1; Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase),; Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1; MGC126826; MGC138389;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P22680 CP7A1_HUMAN:

Detected in liver.

Description:
Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.
Sequence:
MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
88
Horse
88
Sheep
75
Chicken
75
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22680 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
Y176 Phosphorylation
T266 Phosphorylation
S268 Phosphorylation
T269 Phosphorylation
Y432 Phosphorylation
Y433 Phosphorylation
S439 Phosphorylation

Research Backgrounds

Function:

A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Microsome membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in liver.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Oleanolic acid alleviates ANIT-induced cholestatic liver injury by activating Fxr and Nrf2 pathways to ameliorate disordered bile acids homeostasis. PHYTOMEDICINE (PubMed: 35605478) [IF=7.9]

2). NRF2 and FXR dual signaling pathways cooperatively regulate the effects of oleanolic acid on cholestatic liver injury. Phytomedicine (PubMed: 36343550) [IF=7.9]

3). Targeting mTOR/YY1 signaling pathway by quercetin through CYP7A1-mediated cholesterol-to-bile acids conversion alleviated type 2 diabetes mellitus induced hepatic lipid accumulation. Phytomedicine (PubMed: 36889164) [IF=7.9]

4). Antarctic krill peptide alleviated liver fibrosis via downregulating secondary bile acid activated NLRP3 signaling pathway. Food & Function (PubMed: 35762853) [IF=6.1]

5). miR-182-5p promotes hepatocyte-stellate cell crosstalk to facilitate liver regeneration. Communications Biology (PubMed: 35915318) [IF=5.9]

Application: WB    Species: Mouse    Sample: liver

Fig. 7 Hepatic miR-182-5p targets Cyp7a1 to promote hepatocyte proliferation. a The heatmap of DEGs in BA synthesis signaling pathway. Green represents downregulation while red represents up regulation. qRT-PCR analyses of Cyp7a1 and Cyp27a1 gene expression in the liver of miR-182-5p KO (b) or TG mice (c) compared with their respective control mice (3d after PH; n = 4/group). miR-182-5p mimic (182 m) or its negative control (ncm) were overexpressed in primary hepatocytes from C57BL/6 J mice. d, e miR-182-5p level, and the Cyp7a1 and Cyp27a1 genes expression levels were determined by qRT-PCR (n = 5/group). f The Cyp7a1 protein expression level was determined by western blot. g Alignment of the sequences of Cyp7a1 gene promoter and miR-182-5p. h Luciferase reporter assay to examine the interactions between miR-182-5p and the predicted target site in the Cyp7a1 gene promoter. Plasmids with the Cyp7a1 gene promoter or mutated gene promoter were co-transfected with miR-182-5p mimic or control mimic into primary hepatocytes. Renilla luciferase activity was measured by a Dual-Glo luciferase assay system and normalized to internal control firefly luciferase activity (n = 3 biological Replicates). HSCs co-cultured with Cyp7a1 siRNA (HepCyp7a1-siRNA) or their control siRNA-treated (HepNC-siRNA) hepatocytes isolated from TG mice. i, j qRT-PCR analyses of α-SMA and Ihh genes expression in HSCs. k qRT-PCR analyses of Cyclin genes expression in primary hepatocytes. l EdU immunostaining of primary hepatocytes. m A proposed model on the mechanism by which miR-182-5p regulates liver regeneration induced by PH. Error bars in all experiments represent SEM; Significance was determined by unpaired two-tailed Student’s t test and by one-way ANOVA.

6). Effects of Poria cocos extract on metabolic dysfunction-associated fatty liver disease via the FXR/PPARα-SREBPs pathway. Frontiers in Pharmacology (PubMed: 36278226) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 6 EPC ameliorated MAFLD formation in rats by regulating BA metabolism. (A–G) Relative expression of CYP7A1, FXR, CYP27A1, BSEP, CYP7B1, CYP8B1, NTCP mRNA in liver, n = 6; (H–L) Relative expression of protein CYP7A1, FXR, SHP, p-AMPK, and p-ERK in the liver, n = 4; (M–N) Relative expression of protein FXR and FGF15 in the ileum, n = 4. (O–P) Representative immunoblotting images of CYP7A1, FXR, SHP, p-AMPK,and p-ERK in the liver. (Q) Representative immunoblotting images of FXR and FGF15 in the ileum. Data are presented as mean ± SEM. One-way analysis of variance (ANOVA) was conducted for the group comparison. *p < 0.05, **p < 0.01, ***p < 0.001 vs. MOD group. CYP7A1, cholesterol 7α-hydroxylase; FXR, farnesoid X receptor; CYP27A1, sterol 27-hydroxylase; BSEP, bile salt export protein; CYP7B1, oxysterol 7α-hydroxylase; CYP8B1, sterol 12αhydroxylase; NTCP, Na + -taurocholate co-transporting polypeptides; SHP, small heterodimer partner; AMPK, 5’-AMP-activated protein kinase; ERK, Extracellular signal-regulated kinase.

7). An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro. Food and Chemical Toxicology (PubMed: 37080529) [IF=4.3]

8). Effects of Agaricus blazei Murrill polysaccharides on hyperlipidemic rats by regulation of intestinal microflora. Food Science & Nutrition (PubMed: 32566193) [IF=3.9]

Application: WB    Species: rat    Sample: liver

FIGURE 3|Effects of ABP on the protein expression of CYP7A1 and SREBP-1C in rat liver tissue. (a) Relative expression of SREBP-1C protein in the liver of rats; (b) relative expression of CYP7A1 protein in the liver of rats; and(c) electropherogram of hyperlipidemiarelated protein expression in the liver tissue of rats. n = 8; *compared with the NG group, p < .05; **compared with the NG group, p < .01; ##compared with the MG group, p < .01

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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