AKAP3 Antibody - #AF0529

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Product: AKAP3 Antibody
Catalog: AF0529
Description: Rabbit polyclonal antibody to AKAP3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 94kDa; 95kD(Calculated).
Uniprot: O75969
RRID: AB_2834191

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
AKAP3 Antibody detects endogenous levels of total AKAP3.
RRID:
AB_2834191
Cite Format: Affinity Biosciences Cat# AF0529, RRID:AB_2834191.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A kinase (PRKA) anchor protein 3; A-kinase anchor protein 110 kDa; A-kinase anchor protein 3; AKAP 110; AKAP-3; AKAP110; Akap3; AKAP3_HUMAN; Cancer/testis antigen 82; CT82; Fibrous sheath protein of 95 kDa; Fibrousheathin 1; Fibrousheathin I; Fibrousheathin-1; FSP95; PRKA3; protein kinase A binding protein AKAP 110; Protein kinase A-anchoring protein 3; SOB 1; SOB1; Sperm oocyte binding protein 1; Sperm oocyte-binding protein;

Immunogens

Immunogen:
Uniprot:

O75969(AKAP3_HUMAN) >>Visit HPA database.

Gene(ID):
AKAP3(10566)
Expression:
O75969 AKAP3_HUMAN:

Testis specific; only expressed in spermatids.

Description:
May function as a regulator of both motility- and head- associated functions such as capacitation and the acrosome reaction.
Sequence:
MSEKVDWLQSQNGVCKVDVYSPGDNQAQDWKMDTSTDPVRVLSWLRRDLEKSTAEFQDVRFKPGESFGGETSNSGDPHKGFSVDYYNTTTKGTPERLHFEMTHKEIPCQGPRAQLGNGSSVDEVSFYANRLTNLVIAMARKEINEKIDGSENKCVYQSLYMGNEPTPTKSLSKIASELVNETVSACSRNAAPDKAPGSGDRVSGSSQSPPNLKYKSTLKIKESTKERQGPDDKPPSKKSFFYKEVFESRNGDYAREGGRFFPRERKRFRGQERPDDFTASVSEGIMTYANSVVSDMMVSIMKTLKIQVKDTTIATILLKKVLLKHAKEVVSDLIDSFLRNLHSVTGTLMTDTQFVSAVKRTVFSHGSQKATDIMDAMLRKLYNVMFAKKVPEHVRKAQDKAESYSLISMKGMGDPKNRNVNFAMKSETKLREKMYSEPKSEEETCAKTLGEHIIKEGLTLWHKTQQKECKSLGFQHAAFEAPNTQRKPASDISFEYPEDIGNLSLPPYPPEKPENFMYDSDSWAEDLIVSALLLIQYHLAQGGRRDARSFVEAAGTTNFPANEPPVAPDESCLKSAPIVGDQEQAEKKDLRSVFFNFIRNLLSETIFKRDQSPEPKVPEQPVKEDRKLCERPLASSPPRLYEDDETPGALSGLTKMAVSQIDGHMSGQMVEHLMNSVMKLCVIIAKSCDASLAELGDDKSGDASRLTSAFPDSLYECLPAKGTGSAEAVLQNAYQAIHNEMRGTSGQPPEGCAAPTVIVSNHNLTDTVQNKQLQAVLQWVAASELNVPILYFAGDDEGIQEKLLQLSAAAVDKGCSVGEVLQSVLRYEKERQLNEAVGNVTPLQLLDWLMVNL

PTMs - O75969 As Substrate

Site PTM Type Enzyme
T34 Phosphorylation
S35 Phosphorylation
T36 Phosphorylation
S43 Phosphorylation
T102 Phosphorylation
S205 Phosphorylation
S206 Phosphorylation
S208 Phosphorylation
Y214 Phosphorylation
K225 Acetylation
K237 Acetylation
S403 Phosphorylation
Y404 Phosphorylation
T428 Phosphorylation
K433 Ubiquitination
S440 Phosphorylation
S612 Phosphorylation
S635 Phosphorylation
S636 Phosphorylation

Research Backgrounds

Function:

May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.

PTMs:

Phosphorylated on tyrosine residues.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Acrosome.
Note: Ribs of the fibrous sheath in the principal piece of the sperm tail. Dorsal margin of the acrosomal segment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis specific; only expressed in spermatids.

Subunit Structure:

Interacts with ROPN1 AND ROPN1L. Interacts with QRICH2.

Family&Domains:

RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Belongs to the AKAP110 family.

Restrictive clause

 

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