Wnt10b Antibody - #DF9038

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Product: Wnt10b Antibody
Catalog: DF9038
Description: Rabbit polyclonal antibody to Wnt10b
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: O00744
RRID: AB_2842234

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(90%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
Wnt10b Antibody detects endogenous levels of total Wnt10b.
RRID:
AB_2842234
Cite Format: Affinity Biosciences Cat# DF9038, RRID:AB_2842234.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Protein Wnt-10b; Protein Wnt-12; SHFM6; wingless type MMTV integration site family, member 10B; WN10B_HUMAN; WNT 10B protein; WNT 12; Wnt10b; wnt12;

Immunogens

Immunogen:

A synthesized peptide derived from human Wnt10b, corresponding to a region within the internal amino acids.

Uniprot:

O00744(WN10B_HUMAN) >>Visit HPA database.

Gene(ID):
WNT10B(7480)
Expression:
O00744 WN10B_HUMAN:

Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.

Sequence:
MLEEPRPRPPPSGLAGLLFLALCSRALSNEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRNPDVTASALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGKLVSCGCGWKGSGEQDRLRAKLLQLQALSRGKSFPHSLPSPGPGSSPSPGPQDTWEWGGCNHDMDFGEKFSRDFLDSREAPRDIQARMRIHNNRVGRQVVTENLKRKCKCHGTSGSCQFKTCWRAAPEFRAVGAALRERLGRAIFIDTHNRNSGAFQPRLRPRRLSGELVYFEKSPDFCERDPTMGSPGTRGRACNKTSRLLDGCGSLCCGRGHNVLRQTRVERCHCRFHWCCYVLCDECKVTEWVNVCK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
90
Pig
0
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.

PTMs:

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.

Family&Domains:

Belongs to the Wnt family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

References

1). A Highly Bioactive Organic-Inorganic Nanoparticle for Activating Wnt10b Mediated Osteogenesis by Specifically Anchor CCN3 Protein. Advanced healthcare materials, 2025 (PubMed: 39840496) [IF=10.0]
2). Exosome-derived long non-coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β-catenin signalling. Clinical and translational medicine, 2025 (PubMed: 39748192) [IF=7.9]

Application: WB    Species: Mouse    Sample: HFSCs

FIGURE 5 S100A8 was identified as a downstream regulator of AC010789.1 to promote hair follicle stem cells (HFSCs) growth by activating Wnt/β-catenin signalling (A) RNA pull-down analysis of the interaction of S100A8 protein with the AC010789.1 mRNAs in HFSCs. (B) RIP analysis of the endogenous enrichment of AC010789.1 mRNAs in S100A8 protein in HFSCs. (C, D) Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot analysis of the expression levels of S100A8 after the transfection with AC010789.1 OE lentiviruses or si-AC010789.1 into HFSCs. (E, F) qRT-PCR and Western blot analysis of the expression levels of S100A8, K6HF and Lgr5 after the transfection of si-AC010789.1 into HFSCs. (G-I) CCK8, 5-ethynyl-2'-deoxyuridine assay (EdU) and Transwell analysis of the cell proliferation and migration viability after the co-transfection with AC010789.1 OE lentiviruses and si-S100A8 into HFSCs. (J) Western blot analysis of the expression levels of S100A8, Wnt10b, β-catenin and c-myc after the co-transfection with AC010789.1 OE lentiviruses and si-S100A8 into HFSCs. Data shown are the mean ± SEM of three experiments. **p < .01, ***p < .001 and ****p < .0001.
3). Clemastine Ameliorates Perioperative Neurocognitive Disorder in Aged Mice Caused by Anesthesia and Surgery. Frontiers in pharmacology, 2021 (PubMed: 34497527) [IF=5.6]

Application: WB    Species: Mouse    Sample:

FIGURE 4. Effects of clemastine on expression levels of WNT/β-catenin signaling pathway. (A) The differential expression of WNT10B was labeled in our previous sequencing results. (B) Relative mRNA expressions of WNT10B and β-catenin, normalized to that of the GAPDH internal control. (C) Representative western blot images of WNT10B and β-catenin. (D) Relative protein expressions of WNT10B and β-catenin, normalized to that of the β-tubulin internal control. The data are presented as mean ± S.D. (n = 10 mice per group). ***p < 0.005 compared with the CON group. ****p < 0.001 compared with the CON group. #p < 0.05 compared with the PND group. ##p < 0.01 compared with the PND group. ###p < 0.005 compared with the PND group. ####p < 0.001 compared with the PND group.
4). SDF-1 induces directional chemotaxis of BMSCs at the intervertebral fusion site and promotes osteogenic differentiation by regulating Wnt/β-catenin in the bone marrow chimera spinal intervertebral fusion mouse model.. Turkish journal of biology = Turk biyoloji dergisi, 2023 (PubMed: 37529111) [IF=1.1]

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