ATP6V1A Antibody - #DF12353

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Product: ATP6V1A Antibody
Catalog: DF12353
Description: Rabbit polyclonal antibody to ATP6V1A
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 68 kDa; 68kD(Calculated).
Uniprot: P38606
RRID: AB_2845158

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ATP6V1A Antibody detects endogenous levels of total ATP6V1A.
RRID:
AB_2845158
Cite Format: Affinity Biosciences Cat# DF12353, RRID:AB_2845158.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

70kDa subunit; ATP6A1; ATP6V1A; ATP6V1A1; ATPase H+ transporting lysosomal subunit A1; ATPase H+ transporting, lysosomal 70kDa V1 subunit A; H(+) transporting two sector ATPase subunit A; H+ transporting ATPase chain A vacuolar (VA68 type); HO68; OTTHUMP00000214746; V ATPase 69 kDa subunit 1; V ATPase A subunit 1; V ATPase subunit A 1; V-ATPase 69 kDa subunit; V-ATPase subunit A; V-type proton ATPase catalytic subunit A; VA68; Vacuolar ATP synthase catalytic subunit A ubiquitous isoform; Vacuolar ATPase isoform VA68; Vacuolar proton pump alpha subunit 1; Vacuolar proton pump subunit alpha; VATA_HUMAN; Vma1; VPP2;

Immunogens

Immunogen:
Uniprot:

P38606(VATA_HUMAN) >>Visit HPA database.

Gene(ID):
ATP6V1A(523)
Expression:
P38606 VATA_HUMAN:

High expression in the skin.

Sequence:
MDFSKLPKILDEDKESTFGYVHGVSGPVVTACDMAGAAMYELVRVGHSELVGEIIRLEGDMATIQVYEETSGVSVGDPVLRTGKPLSVELGPGIMGAIFDGIQRPLSDISSQTQSIYIPRGVNVSALSRDIKWDFTPCKNLRVGSHITGGDIYGIVSENSLIKHKIMLPPRNRGTVTYIAPPGNYDTSDVVLELEFEGVKEKFTMVQVWPVRQVRPVTEKLPANHPLLTGQRVLDALFPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38606 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
K8 Ubiquitination
K84 Ubiquitination
Y117 Phosphorylation
S125 Phosphorylation
K132 Ubiquitination
T136 Phosphorylation
K139 Ubiquitination
S145 Phosphorylation
T148 Phosphorylation
Y153 Phosphorylation
S160 Phosphorylation
K163 Ubiquitination
K165 Ubiquitination
T175 Phosphorylation
K220 Ubiquitination
K256 Ubiquitination
K265 Methylation
K265 Ubiquitination
K307 Ubiquitination
T309 Phosphorylation
S316 Phosphorylation
R364 Methylation
R381 Methylation
S384 Phosphorylation
K393 Ubiquitination
Y454 Phosphorylation
Y457 Phosphorylation
Y464 Phosphorylation
Y465 Phosphorylation
K467 Acetylation
K467 Ubiquitination
K480 Ubiquitination
K498 Ubiquitination
K506 Ubiquitination
K513 Ubiquitination
K516 Ubiquitination
Y528 Phosphorylation
R530 Methylation
K564 Ubiquitination
Y579 Phosphorylation
K580 Acetylation
K580 Ubiquitination
K585 Ubiquitination
K587 Ubiquitination
K591 Ubiquitination
K596 Acetylation
K598 Ubiquitination
S599 Phosphorylation
Y601 Phosphorylation
S614 Phosphorylation

Research Backgrounds

Function:

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

High expression in the skin.

Subunit Structure:

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).

Family&Domains:

Belongs to the ATPase alpha/beta chains family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Collecting duct acid secretion.

Restrictive clause

 

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