Product: GRF-1 Antibody
Catalog: AF6484
Description: Rabbit polyclonal antibody to GRF-1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 155kDa; 171kD(Calculated).
Uniprot: Q9NRY4
RRID: AB_2835164

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
GRF-1 Antibody detects endogenous levels of total GRF-1.
Cite Format: Affinity Biosciences Cat# AF6484, RRID:AB_2835164.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


ARHGAP35; GAP associated protein p190; Glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF 1; GRF-1; GRF1; GRLF 1; GRLF1; GRLF1_HUMAN; KIAA1722; MGC10745; p190 A; p190-A; P190A; P190A, rat, homolog of; p190ARhoGAP; p190RhoGAP; Rho GAP p190A; Rho GTPase-activating protein 35;



Detected in neutrophils (at protein level).

GRF-1 the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP).



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRY4 As Substrate

Site PTM Type Enzyme
S46 Phosphorylation
Y133 Phosphorylation
T136 Phosphorylation
S182 Phosphorylation
K193 Acetylation
Y209 Phosphorylation
S296 Phosphorylation
Y308 Phosphorylation
K368 Ubiquitination
S575 Phosphorylation
S576 Phosphorylation
S589 Phosphorylation
S591 Phosphorylation
S667 Phosphorylation
T685 Phosphorylation
K762 Ubiquitination
S769 Phosphorylation
S770 Phosphorylation
T771 Phosphorylation
S773 Phosphorylation
Y855 Phosphorylation
T906 Phosphorylation
S962 Phosphorylation
T963 Phosphorylation
S970 Phosphorylation
S975 Phosphorylation
S980 Phosphorylation
S985 Phosphorylation
S1001 Phosphorylation
S1004 Phosphorylation
S1006 Phosphorylation
S1010 Phosphorylation
S1023 Phosphorylation
S1057 Phosphorylation
S1070 Phosphorylation
S1072 Phosphorylation
S1073 Phosphorylation
S1074 Phosphorylation
S1085 Phosphorylation
Y1087 Phosphorylation P06241 (FYN)
K1096 Ubiquitination
Y1105 Phosphorylation Q13882 (PTK6) , P12931 (SRC) , P42684 (ABL2) , P06241 (FYN)
S1106 Phosphorylation
S1111 Phosphorylation
T1112 Phosphorylation
K1115 Ubiquitination
S1127 Phosphorylation
S1130 Phosphorylation
S1134 Phosphorylation
S1136 Phosphorylation
T1140 Phosphorylation
S1141 Phosphorylation
S1142 Phosphorylation
S1150 Phosphorylation Q13464 (ROCK1)
S1153 Phosphorylation
Y1158 Phosphorylation
T1173 Phosphorylation Q13464 (ROCK1)
S1174 Phosphorylation Q13464 (ROCK1)
S1176 Phosphorylation
S1179 Phosphorylation
S1195 Phosphorylation
Y1198 Phosphorylation
Y1207 Phosphorylation
T1209 Phosphorylation
S1221 Phosphorylation P17252 (PRKCA)
T1226 Phosphorylation Q13464 (ROCK1) , P17252 (PRKCA)
S1236 Phosphorylation Q13464 (ROCK1) , P17252 (PRKCA)
K1289 Ubiquitination
S1290 Phosphorylation
K1353 Ubiquitination
K1358 Ubiquitination
K1381 Ubiquitination
S1450 Phosphorylation
S1476 Phosphorylation
T1480 Phosphorylation
S1491 Phosphorylation

Research Backgrounds


Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences.


Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I. Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids. In brain, phosphorylated by FYN and SRC (By similarity). During focal adhesion formation, phosphorylated by MAPK1 and MAPK3 at the C-terminal region, probably at Ser-1451, Ser-1476, Thr-1480 and Ser-1483. Phosphorylation by MAPK1 and MAPK3 inhibits GAP function and localizes ARGHAP35 away from newly forming focal adhesions and stress fibers in cells spreading on fibronectin (By similarity). Phosphorylation at Ser-1476 and Thr-1480 by GSK3B requires priming by MAPK and inhibits RhoGAP activity and modulates polarized cell migration (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm. Nucleus. Cell membrane.
Note: In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in neutrophils (at protein level).

Subunit Structure:

Interacts with RASA1 (By similarity). Interacts with the general transcription factor GTF2I, the interaction sequesters GTF2I in the cytoplasm.


N-terminal part (1-266) has GTPase activity. Required for proper cellular localization.

The pG1 pseudoGTPase domain does not bind GTP.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

Restrictive clause


Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.