Product: RPS6 Antibody
Catalog: AF6354
Description: Rabbit polyclonal antibody to RPS6
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 32kDa; 29kD(Calculated).
Uniprot: P62753
RRID: AB_2835204

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
RPS6 Antibody detects endogenous levels of total RPS6.
Cite Format: Affinity Biosciences Cat# AF6354, RRID:AB_2835204.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;


Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62753 As Substrate

Site PTM Type Enzyme
K2 Methylation
S6 Phosphorylation
C12 S-Nitrosylation
K14 Acetylation
K14 Ubiquitination
R22 Methylation
K23 Ubiquitination
Y28 Phosphorylation
K30 Acetylation
K30 Ubiquitination
K46 Ubiquitination
S53 Phosphorylation
K58 Ubiquitination
K64 Ubiquitination
T69 Phosphorylation
S78 Phosphorylation
K79 Acetylation
K79 Ubiquitination
S82 Phosphorylation
K116 Ubiquitination
K119 Ubiquitination
T128 Phosphorylation
K143 Ubiquitination
S148 Phosphorylation
K149 Ubiquitination
R154 Methylation
K160 Ubiquitination
T181 Phosphorylation
K203 Ubiquitination
Y209 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K218 Acetylation
S235 Phosphorylation Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1)
S236 Phosphorylation Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK)
S240 Phosphorylation Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1)
T241 Phosphorylation
S242 Phosphorylation Q13177 (PAK2)
S244 Phosphorylation Q15418 (RPS6KA1) , P23443 (RPS6KB1)
S246 Phosphorylation
S247 Phosphorylation P23443 (RPS6KB1)

Research Backgrounds


May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.


Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.


Belongs to the eukaryotic ribosomal protein eS6 family.

Research Fields

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome.

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)


1). Wang L et al. Activation of integrated stress response and disordered iron homeostasis upon combined exposure to cadmium and PCB77. JOURNAL OF HAZARDOUS MATERIALS 2019 Dec 5:121833 (PubMed: 31837937) [IF=13.6]

Application: WB    Species: Human    Sample: Human erythroleukemia cell lines (HEL)

Fig. 5. Disordered iron homeostasis and inhibited mTORC1 activity upon exposure to CdCl2 and PCB77 at low dose. (A) The relative fluorescence intensity of CAeAM for measuring LIP to reflect intracellular iron availability (n = 3–4), and (B) Representative blots of FTH1 protein content to reflect iron storage. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. (C) Phosphorylated S6 and total S6 content to re- flect mTORC1 activity as measured by Western blot. Ratio of FTH1 to eIF2αP and ratio of pS6 to S6 in the control group were defined as 1. Analyses were performed after single or combined exposure to CdCl2 and PCB77 at 1 μM for 48 h. a- significantly different from the control group. Data were presented in mean ± SE. P < 0.05 was considered statistically significant.

2). Li X et al. Bergenin attenuates bleomycin‐induced pulmonary fibrosis in mice via inhibiting TGF‐β1 signaling pathway. PHYTOTHERAPY RESEARCH 2021 Aug 10. (PubMed: 34375009) [IF=7.2]

Application: WB    Species: Mice    Sample: NIH-3 T3 cells

FIGURE 6 Bergenin promotes fibroblasts autophagy mainly via inhibiting mTOR signaling pathway. Mlg and NIH-3 T3 cells were co-treated with bergenin (30 and 60 μM) and TGF-β1 (5 ngml1 ) for 24 h. (a,b) The expression of p-mTOR, mTOR, p-ULK1, ULK1, p-S6, and S6 in Mlg cells (a) and NIH-3 T3 cells (b). GAPDH was used as the internal control. Data are presented as the means ± SD. Experiments were performed in triplicate (n = 3). *p < .05; **p < .01; ***p < .001

3). Liao W et al. Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening. Frontiers in Pharmacology 2021 Oct 29;12:658197. (PubMed: 34776939) [IF=5.6]

Application: WB    Species: Human    Sample: HCC Cells

FIGURE 5 Western blotting of downstream regulated proteins of CUDC-907 and immunohistochemistry staining. (A) The expression of c-Myc is regulated by the HDAC and PI3K/AKT/mTOR pathway, and both can be inhibited by CUDC-907. (B) Expression levels of proteins [c-Myc, p-AKT, p-S6, p-4EBP1, and acetyl histone H3K9 (H3K9ac)] in HCC cell lines affected by CUDC-907 were detected by western blotting. GAPDH was set as the internal reference. (C) Expression levels of proteins (same as figure 5B) from xenografted tumor with or without CUDC-907 treatment were detected. (D) Immunohistochemistry staining. Representative images of Ki67, c-Myc, and TUNEL staining of xenografts treated with CUDC-907 or vehicle. (E) Quantification of immunohistochemistry staining was calculated by the immunohistochemistry score method. The percentage of apoptotic area in tumor slides of TUNEL staining was calculated by IMAGE J software. *p < 0.05, **p < 0.001 (result compared with Vehicle group).

4). You Y et al. Ketogenic diet aggravates cardiac remodeling in adult spontaneously hypertensive rats. Nutrition & Metabolism 2020 Oct 26;17:91. (PubMed: 33117428) [IF=4.5]

Application: WB    Species: Rat    Sample: heart tissue

Fig. 5 Ketogenic diet activated mTORC2 in the heart of spontaneously hypertensive rats. a The phosphorylation of AMPK and the ratio of p-S6/S6 and p-Akt/Akt in the heart of Wistar rats and SHRs were measured with Western blots. The quantitative result were shown in b–d. Values are the mean ± SEM. n = 4 for each group. *P < 0.05, **P < 0.01

5). Zhang JQ et al. Autophagy Contributes to Oxidative Stress-Induced Apoptosis in Porcine Granulosa Cells. Reproductive Sciences 2020 Oct 20. (PubMed: 33079330) [IF=2.9]

Application: WB    Species: Porcine    Sample: granulosa cell (GC)

Fig. 5 H2O2 induces GC autophagy through the mTOR and Ras-cAMPPKA signaling pathway. Primary cultured GC were treated with H2O2 (200 μmol/l) for different time periods. Then, the GC samples were analyzed by immunoblotting using the corresponding antibody. a The expression of mTOR protein was determined by western blotting. b The expression of PI3K, AKT, and RPS6 was determined by western blotting.c The expression of Ras, cAMP, and PKA was determined by western blotting. d–j The relative expression of p-mTOR, p-PI3K, p-AKT, pRPS6, Ras, cAMP, and PKA were quantified by software Image J. Data represent as the means ± SE from three independent experiments. * p < 0.05, ** p < 0.01

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