ATP1B1 Recombinant Rabbit mAb - #BF4079

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Product: ATP1B1 Recombinant Rabbit mAb
Catalog: BF4079
Description: Rabbit monoclonal antibody to ATP1B1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 45-55 kDa(Observed); 35kD(Calculated).
Uniprot: P05026

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Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human, Mouse, Rat
Clonality:
Monoclonal [ReFirm23526]
Specificity:
ATP1B1 Recombinant Rabbit mAb detects endogenous levels of ATP1B1.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP1B

Immunogens

Immunogen:

A synthetic peptide from human ATP1B1

Uniprot:

P05026(AT1B1_HUMAN) >>Visit HPA database.

Gene(ID):
ATP1B1(481)
Expression:
P05026 AT1B1_HUMAN:

Found in most tissues.

Description:
The protein encoded by this gene belongs to the family of Na+,K+ and H+,K+ ATPases beta chain proteins,and to the subfamily of Na+,K+ -ATPases. Na+,K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation,for sodium-coupled transport of a variety of organic and inorganic molecules,and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits,a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates,through assembly of alpha,beta heterodimers,the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+,K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described,but their biological validity is not known.
Sequence:
MARGKAKEEGSWKKFIWNSEKKEFLGRTGGSWFKILLFYVIFYGCLAGIFIGTIQVMLLTISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS

Research Backgrounds

Function:

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

Involved in cell adhesion and establishing epithelial cell polarity.

PTMs:

Glutathionylated.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein. Cell membrane>Sarcolemma.
Note: Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in most tissues.

Family&Domains:

The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.

Belongs to the X(+)/potassium ATPases subunit beta family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Protein digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

Restrictive clause

 

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For Research Use Only.
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