R-Ras Recombinant Rabbit mAb - #BF4478

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Product: R-Ras Recombinant Rabbit mAb
Catalog: BF4478
Description: Rabbit monoclonal antibody to R-Ras
Application: WB
Reactivity: Human
Mol.Wt.: 24 kDa(Observed); 23kD(Calculated).
Uniprot: P10301

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit IgG
Application:
WB 1:1000-1:5000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Monoclonal [ReFirm23928]
Specificity:
R-Ras Recombinant Rabbit mAb detects endogenous levels of R-Ras.
Conjugate:
Unconjugated.
Purification:
Affinity-chromatography.
Storage:
Rabbit IgG in Tris-Glycine (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Oncogene RRAS; p23; R Ras; Ras related Protein; Ras related protein R Ras; Ras-related protein R-Ras; related RAS viral (r ras) oncogene homolog; RRAS; RRAS_HUMAN;

Immunogens

Immunogen:

A synthetic peptide from human R-Ras

Uniprot:

P10301(RRAS_HUMAN) >>Visit HPA database.

Gene(ID):
RRAS(6237)
Description:
The protein encoded by this gene is a small GTPase involved in diverse processes including angiogenesis,vascular homeostasis and regeneration,cell adhesion,and neuronal axon guidance. Mutations in this gene are found in many invasive cancers.
Sequence:
MSSGAASGTGRGRPRGGGPGPGDPPPSETHKLVVVGGGGVGKSALTIQFIQSYFVSDYDPTIEDSYTKICSVDGIPARLDILDTAGQEEFGAMREQYMRAGHGFLLVFAINDRQSFNEVGKLFTQILRVKDRDDFPVVLVGNKADLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPSPPSAPRKKGGGCPCVLL

Research Backgrounds

Function:

Regulates the organization of the actin cytoskeleton. With OSPBL3, modulates integrin beta-1 (ITGB1) activity.

PTMs:

S-palmitoylated by ZDHHC19, leading to increased association with membranes and with rafts/caveolae as well as enhanced cell viability.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.
Note: Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the small GTPase superfamily. Ras family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Development > Axon guidance.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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