Integrin alpha 5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells, using Integrin alpha 5 (CD49e) Antibody.

AF5200 at 1/100 staining Mouse heart tissue by IHC-P.

AF5200 at 1/100 staining Rat heart tissue by IHC-P.

Product:	Integrin alpha 5 Antibody
Catalog:	AF5200
Description:	Rabbit polyclonal antibody to Integrin alpha 5
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Rabbit, Dog
Mol.Wt.:	114kDa,150kDa; 115kD(Calculated).
Uniprot:	P08648
RRID:	AB_2837686

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Integrin alpha 5 Antibody detects endogenous levels of total Integrin alpha 5.

RRID:

AB_2837686
Cite Format: Affinity Biosciences Cat# AF5200, RRID:AB_2837686.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; Fibronectin receptor, alpha subunit; FNRA; Integrin alpha 5 (fibronectin receptor alpha); Integrin alpha-5; Integrin alpha-5 light chain; Integrin alpha-F; Integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITA5_HUMAN; Itga5; Very late activation protein 5, alpha subunit; VLA-5; VLA5; VLA5A;

Immunogens

Immunogen:

Uniprot:

P08648(ITA5_HUMAN) >>Visit HPA database.

Gene(ID):

ITGA5(3678)

Description:

Integrins are heterodimers composed of noncovalently associated transmembrane α and β subunits. The 16 α and 8 β subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and Vitronectin.

Sequence:

P08648 ITA5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Pig

Bovine

Sheep

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08648 As Substrate

P08648

Site	PTM Type	Source
N84	N-Glycosylation	Uniprot
S123	Phosphorylation	Uniprot
S124	Phosphorylation	Uniprot
S126	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
S128	Phosphorylation	Uniprot
K137	Ubiquitination	Uniprot
K166	Ubiquitination	Uniprot
N182	N-Glycosylation	Uniprot
S210	Phosphorylation	Uniprot
N297	N-Glycosylation	Uniprot
N307	N-Glycosylation	Uniprot
N316	N-Glycosylation	Uniprot
S394	Phosphorylation	Uniprot
S395	Phosphorylation	Uniprot
Y487	Phosphorylation	Uniprot
N609	N-Glycosylation	Uniprot
N675	N-Glycosylation	Uniprot
C731	S-Nitrosylation	Uniprot
S742	Phosphorylation	Uniprot
N773	N-Glycosylation	Uniprot
S887	Phosphorylation	Uniprot
K943	Ubiquitination	Uniprot
K1042	Ubiquitination	Uniprot
S1047	Phosphorylation	Uniprot

Research Backgrounds

P08648_ITA5_HUMAN

Function:

Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling.

(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.

(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.

(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

PTMs:

Proteolytic cleavage by PCSK5 mediates activation of the precursor.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion. Cell surface.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with CIB1. ITGA5:ITGB1 interacts with CCN3. ITGA5:ITGB1 interacts with FBN1. ITGA5:ITGB1 interacts with IL1B.

(Microbial infection) Integrin ITGA5:ITGB1 interacts with human metapneumovirus fusion protein.

(Microbial infection) Integrin ITGA2:ITGB1 interacts with human parvovirus B19 capsid proteins.

(Microbial infection) Interacts with HIV-1 Tat.

Family&Domains:

Belongs to the integrin alpha chain family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

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Integrin alpha 5 Antibody - #AF5200