Product: Wnt1 Antibody
Catalog: AF5315
Description: Rabbit polyclonal antibody to Wnt1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 41 kDa; 41kD(Calculated).
Uniprot: P04628
RRID: AB_2837800

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Wnt1 Antibody detects endogenous levels of total Wnt1.
Cite Format: Affinity Biosciences Cat# AF5315, RRID:AB_2837800.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


BMND16; INT1; OI15; oncogene Int1; Proto oncogene protein Wnt 1; Proto-oncogene Int-1 homolog; Proto-oncogene Wnt-1; Wingless type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1); Wnt 1; wnt1; WNT1_HUMAN;


Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.



Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04628 As Substrate

Site PTM Type Enzyme
S132 Phosphorylation
T136 Phosphorylation
S144 Phosphorylation
T348 Phosphorylation
S356 Phosphorylation

Research Backgrounds


Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis.


Palmitoleoylation is required for efficient binding to frizzled receptors. Palmitoleoylation is necessary for proper trafficking to cell surface (Probable). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway (By similarity).

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids. Interacts with PORCN. Interacts with RSPO1, RSPO2 and RSPO3 (By similarity). Interacts with WLS (By similarity).


Belongs to the Wnt family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.


1). XQ-1H alleviates cerebral ischemia in mice through inhibition of apoptosis and promotion of neurogenesis in a Wnt/β-catenin signaling dependent way. LIFE SCIENCES, 2019 (PubMed: 31499069) [IF=6.1]

2). Yanghe Decoction Suppresses the Experimental Autoimmune Thyroiditis in Rats by Improving NLRP3 Inflammasome and Immune Dysregulation. Frontiers in Pharmacology, 2021 (PubMed: 34234669) [IF=5.6]

Application: WB    Species: Rat    Sample: peripheral blood mononuclear cell (PBMCs)

FIGURE 7 RT-PCR and Western blot analysis for the expression of molecules in the Wnt/β-catenin signaling pathway in peripheral blood mononuclear cell (PBMCs) (A) mRNA expression of Wnt-1 and β-catenin (B) Western blotting images of Wnt-1and β-catenin: Lane 1, control group; lane 2, model group; lane 3, YH 5 g crude drug/kG group; lane 4, YH 15 g crude drug/kG group; lane 5, selenious group (C) Bar graphs indicate the relative ratio of Wnt-1and β-catenin over tubulin. All values are expressed as mean ± SEM. *p < 0.05, **p < 0.01 vs Model.

3). Phillygenin Inhibits TGF-β1-induced Hepatic Stellate Cell Activation and Inflammation: Regulation of the Bax/Bcl-2 and Wnt/β-catenin Pathways. Inflammation, 2024 (PubMed: 38393550) [IF=5.1]

Application: WB    Species: Mouse    Sample: mHSC

Fig. 10 Efects of PHI on Wnt/β-catenin signaling pathway-related proteins. a Western blotting analyses of Wnt1, β-catenin, GSK-3β, p-GSK-3β, c-Myc, and Cyclin D1 in mHSCs.

4). Adipose derived mesenchymal stem cells alleviated osteoarthritis and chondrocyte apoptosis through autophagy inducing. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 30315711) [IF=4.0]

5). Indoxyl sulfate promotes the atherosclerosis through up-regulating the miR-34a expression in endothelial cells and vascular smooth muscle cells in vitro. VASCULAR PHARMACOLOGY, 2020 (PubMed: 32593718) [IF=4.0]

Application: WB    Species: human    Sample: HUVECs and HA-VSMCs

Fig. 7.| IS inhibits the Notch signaling pathway and miR-34a-related protein. After transfection with miR-34a mimics (50 nM) or inhibitor (100 nM), IS (1000 μM) was added and incubated for another 36 h. Western blotting was used to detected the protein expression in HUVECs and HA-VSMCs. Data are represented as the mean ± SEM (n = 3). ⁎P < .05, versus control, #P < .05, versus IS group by one-way ANOVA with Tukey's test

6). Tangshen Formula Improves Diabetes-Associated Myocardial Fibrosis by Inhibiting TGF-β/Smads and Wnt/β-Catenin Pathways. Frontiers in Medicine, 2021 (PubMed: 34938743) [IF=3.9]

Application: WB    Species: Mouse    Sample:

Figure 4 An effect of TSF on the canonical Wnt pathway. (A) IHC of anti-β-catenin (original magnification, 200×). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 TSF-treated group vs. KKAy group; #p < 0.05, ##p < 0.01, ###p < 0.001 KKAy group vs. C57BL/6J group. (B) Western blot analyses and semi-quantitative analyses revealed expressions of Wnt1, active-β-catenin, β-catenin, FN, and MMP-7 in KKAy mice and the different treatment groups. #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 KKAy group vs. C57BL/6J group. *p < 0.05, **p < 0.01 TSF-treated group vs. KKAy group.

7). Chemokine (C-C motif) ligand 2-enhanced adipogenesis and angiogenesis of human adipose-derived stem cell and human umbilical vein endothelial cell co-culture system in adipose tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 2022 (PubMed: 34811942) [IF=3.3]

8). Neoisoliquiritigenin Inhibits Tumor Progression by Targeting GRP78-β- catenin Signaling in Breast Cancer. CURRENT CANCER DRUG TARGETS, 2018 (PubMed: 28914191) [IF=3.0]

9). Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells. Experimental and Therapeutic Medicine, 2020 (PubMed: 32256772) [IF=2.7]

Application: WB    Species: Human    Sample: SW1353 cells

Figure 4 Effect of irisin on the activation of the Wnt/β-catenin signaling pathway in IL-1β-induced SW1353 cells. SW1353 cells were treated with 20 mM irisin for 24 h, with or without IL-1β, and (A) β-catenin protein levels, (B) Wnt-1 protein levels and (C) β-catenin and Wnt-1 protein expression,were evaluated by western blot analysis. n=5/group. mRNA expression levels of (D) β-catenin and (E) Wnt-1 were evaluated by reverse transcription-quantitative PCR. n=5/group. *P<0.05, **P<0.01 and ***P<0.001. IL-1β, interleukin-1β; NC, negative control.

10). HDAC1 regulates inflammation and osteogenic differentiation of ankylosing spondylitis fibroblasts through the Wnt-Smad signaling pathway. Journal of Orthopaedic Surgery and Research, 2022 (PubMed: 35794630) [IF=2.6]

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