Product: CSF1 Antibody
Catalog: BF0475
Description: Mouse monoclonal antibody to CSF1
Application: WB IHC ELISA
Reactivity: Human
Mol.Wt.: 60kDa; 60kD(Calculated).
Uniprot: P09603
RRID: AB_2833766

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 200ul $450 In stock

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Product Info

ELISA 1:10000, WB 1:500-1:2000, IHC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1742]
CSF1 antibody detects endogenous levels of total CSF1.
Cite Format: Affinity Biosciences Cat# BF0475, RRID:AB_2833766.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Colony stimulating factor 1 (macrophage); Colony stimulating factor 1; Colony stimulating factor macrophage specific; CSF 1; CSF-1; CSF1; CSF1_HUMAN; Csfm; Lanimostim; M CSF; M-CSF; Macrophage Colony Stimulating Factor 1; Macrophage colony stimulating factor; MCSF; MGC31930; Processed macrophage colony-stimulating factor 1;



Purified recombinant fragment of human CSF1 expressed in E. Coli.

CSF1, also known as MCSF, is a four-α-helicalbundle cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. This protein may be involved in development of the placenta.

PTMs - P09603 As Substrate

Site PTM Type Enzyme
S256 O-Glycosylation
T257 O-Glycosylation
T266 O-Glycosylation
T266 Phosphorylation
S345 Phosphorylation
S348 Phosphorylation
S353 Phosphorylation
T363 O-Glycosylation
T365 O-Glycosylation
T376 O-Glycosylation
T383 O-Glycosylation
T387 O-Glycosylation
S391 O-Glycosylation
S420 Phosphorylation
S426 Phosphorylation
S428 O-Glycosylation
S430 O-Glycosylation
S431 O-Glycosylation
S433 O-Glycosylation
S451 O-Glycosylation
S461 O-Glycosylation
S461 Phosphorylation
S473 O-Glycosylation
T477 O-Glycosylation
S533 Phosphorylation

Research Backgrounds


Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.


N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer or heterodimer; disulfide-linked. Interacts with CSF1R.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

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