Phospho-IRS2 (Ser1100) Antibody | Affinity Biosciences

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Phospho-IRS2 (Ser1100) Antibody - Western blot analysis of extracts from Hela cells(heat-shock treatment), using Phospho-IRS2 (Ser1100) Antibody.

Phospho-IRS2 (Ser1100) Antibody - AF8383 at 1/100 staining Human pancreatic cancer by IHC-P.

Phospho-IRS2 (Ser1100) Antibody - AF8383 staining Hela cells(heat shock treatment) by IF/ICC.

Phospho-IRS2 (Ser1100) Antibody - peptide-ELISA analysis of AF8383.

Phospho-IRS2 (Ser1100) Antibody - FIGURE 4 | Sleep restriction induced depletion of circadian clock expression.

Phospho-IRS2 (Ser1100) Antibody - FIGURE 11 Involvement of SHBG in the insulin signaling pathway.

Product:	Phospho-IRS2 (Ser1100) Antibody
Catalog:	AF8383
Description:	Rabbit polyclonal antibody to Phospho-IRS2 (Ser1100)
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse
Prediction:	Dog
Mol.Wt.:	137kDa; 137kD(Calculated).
Uniprot:	Q9Y4H2
RRID:	AB_2840444

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Dog(89%)

Clonality:

Polyclonal

Specificity:

Phospho-IRS2 (Ser1100) Antibody detects endogenous levels of IRS2 only when phosphorylated at Ser1100.

RRID:

AB_2840444
Cite Format: Affinity Biosciences Cat# AF8383, RRID:AB_2840444.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Insulin receptor substrate 2; IRS 2; IRS-2; Irs2; IRS2_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human IRS2 around the phosphorylation site of Ser1100.

Uniprot:

Q9Y4H2(IRS2_HUMAN) >>Visit HPA database.

Gene(ID):

IRS2(8660)

Sequence:

Q9Y4H2 IRS2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTYASIDFLSHHLKEATIVKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9Y4H2_IRS2_HUMAN

Function:

May mediate the control of various cellular processes by insulin.

Subcellular Location:

Cytoplasm>Cytosol.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences, 2023 (PubMed: 38146533) [IF=5.0]

Application: WB Species: Human Sample: adipose tissue

FIGURE 11 Involvement of SHBG in the insulin signaling pathway. Relative expression levels of PI3K (A), AKT1 (B), GLUT4 (C), IRS1 (D), IRS2 (E), INSR (F) were analyzed by qRT-PCR. Protein levels of PI3K (G), AKT (H), GLUT4 (I), IRS1 (J), IRS2 (K), SHBG (L), IR (M) were analyzed by Western Blot. Representative immunoblots (N). Representative data are shown as mean ± SD.

2). Chronic timed sleep restriction attenuates LepRb-mediated signaling pathways and circadian clock gene expression in the rat hypothalamus. Frontiers in Neuroscience, 2020 (PubMed: 33013300) [IF=4.3]

Application: WB Species: rat Sample:

FIGURE 4 | Sleep restriction induced depletion of circadian clock expression. (A) Western blot analysis. Images are representative of five independent experiments for each group.

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Phospho-IRS2 (Ser1100) Antibody - #AF8383