IRS2 Antibody | Affinity Biosciences

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IRS2 Antibody - FIGURE 4 | Sleep restriction induced depletion of circadian clock expression.

IRS2 Antibody - Figure 11 Effect of YQ on insulin secretion-related proteins in pancreas islet.

Product:	IRS2 Antibody
Catalog:	DF7534
Description:	Rabbit polyclonal antibody to IRS2
Application:	WB IHC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	185 kDa; 137kD(Calculated).
Uniprot:	Q9Y4H2
RRID:	AB_2841033

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(100%)

Clonality:

Polyclonal

Specificity:

IRS2 Antibody detects endogenous levels of total IRS2.

RRID:

AB_2841033
Cite Format: Affinity Biosciences Cat# DF7534, RRID:AB_2841033.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Insulin receptor substrate 2; IRS 2; IRS-2; Irs2; IRS2_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human IRS2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9Y4H2(IRS2_HUMAN) >>Visit HPA database.

Gene(ID):

IRS2(8660)

Sequence:

Q9Y4H2 IRS2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTYASIDFLSHHLKEATIVKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9Y4H2_IRS2_HUMAN

Function:

May mediate the control of various cellular processes by insulin.

Subcellular Location:

Cytoplasm>Cytosol.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Methyl ferulic acid ameliorates alcohol-induced hepatic insulin resistance via miR-378b-mediated activation of PI3K-AKT pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 34844105) [IF=6.9]

2). Highland Barley Alleviates High-Fat Diet-Induced Obesity and Liver Injury Through the IRS2/PI3K/AKT Signaling Pathway in Rats. Nutrients, 2024 (PubMed: 39458511) [IF=5.9]

3). Chronic timed sleep restriction attenuates LepRb-mediated signaling pathways and circadian clock gene expression in the rat hypothalamus. Frontiers in Neuroscience, 2020 (PubMed: 33013300) [IF=4.3]

Application: WB Species: rat Sample:

FIGURE 4 | Sleep restriction induced depletion of circadian clock expression. (A) Western blot analysis. Images are representative of five independent experiments for each group.

4). YiQi YangYin Decoction Attenuates Nonalcoholic Fatty Liver Disease in Type 2 Diabetes Rats. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 34621322)

Application: WB Species: Rat Sample: The liver and pancreas tissues

Figure 11 Effect of YQ on insulin secretion-related proteins in pancreas islet. The expressions levels of IRS-2, P-PI3K, PI3K, P-Akt, Akt, and GLUT4 were assessed by western blot analysis. The results are shown as means ± SEM (n = 9–10). ##P < 0.01 compared with the control group; ∗P < 0.05, ∗∗P < 0.01 compared with the model group.

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For Research Use Only.
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IRS2 Antibody - #DF7534