Product: FGF2 Antibody
Catalog: DF6038
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 21kD; 31kD(Calculated).
Uniprot: P09038
RRID: AB_2838011

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
FGF2 Antibody detects endogenous levels of total FGF2.
RRID:
AB_2838011
Cite Format: Affinity Biosciences Cat# DF6038, RRID:AB_2838011.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basic fibroblast growth factor; Basic fibroblast growth factor bFGF; BFGF; FGF 2; FGF B; FGF-2; Fgf2; FGF2 basic; FGF2_HUMAN; FGFB; Fibroblast growth factor 2 (basic); Fibroblast growth factor 2; Fibroblast growth factor, basic; HBGF 2; HBGF-2; HBGF2; HBGH 2; HBGH2; Heparin binding growth factor 2 precursor; Heparin-binding growth factor 2; Prostatropin;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P09038 FGF2_HUMAN:

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Description:
Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, 4 receptors and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS-2, PLC and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similiar biological activities with quantitative differences likely due to variation in receptor affinity or binding (7).
Sequence:
MVGVGGGDVEDVTPRPGGCQISGRGARGCNGIPGAAAWEAALPRRRPRRHPSVNPRSRAAGSPRTRGRRTEERPSGSRLGDRGRGRALPGGRLGGRGRGRAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

PTMs - P09038 As Substrate

Site PTM Type Enzyme
S62 Phosphorylation
T65 Phosphorylation
R82 Methylation
R84 Methylation
R86 Methylation
R96 Methylation
R98 Methylation
R108 Methylation
R110 Methylation
R112 Methylation
R118 Methylation
R124 Methylation
S138 Phosphorylation
T141 Phosphorylation
K168 Acetylation
S206 Phosphorylation
Y215 Phosphorylation P42680 (TEC)
K228 Sumoylation
K228 Ubiquitination
S242 Phosphorylation
Y245 Phosphorylation
S250 Phosphorylation
Y253 Phosphorylation
T254 Phosphorylation P17612 (PRKACA)
Y257 Phosphorylation P42680 (TEC)
K261 Acetylation
K267 Acetylation
K267 Ubiquitination
K271 Acetylation
K271 Ubiquitination
K277 Acetylation
S285 Phosphorylation
S288 Phosphorylation

Research Backgrounds

Function:

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis.

PTMs:

Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.

Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

Subcellular Location:

Secreted. Nucleus.
Note: Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Subunit Structure:

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP3. Interacts with integrin ITGAV:ITGB3; the interaction is required for FGF2 signaling. Interacts with SNORC (via the extracellular domain) (By similarity). Interacts with glypican GPC3 (By similarity).

Family&Domains:

Belongs to the heparin-binding growth factors family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). Li S et al. Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY) 2020 Jun 9;12. (PubMed: 32518214) [IF=5.955]

Application: IF/ICC    Species: rat    Sample:

Figure 6. | Sal upregulates the FGF2-mediated cAMP/PKA/CREB signaling pathway following MCAO/R.(A–G) Representative western blot bands of cAMP, PKA, CREB, p-CREB, FGF2 and FGFR1 in each group. (H–K) QPCR analysis of PKA, p-CREB, FGF2 and FGFR1 mRNA expression at 7 days after MCAO/R in different groups. (I) Double staining for FGF2-positive (green) and NeuN-positive neurons (red) neurons(the scale bars are 20 μm and 10 μm). Values are expressed as the mean ± SD. #p < 0.05, ##p < 0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.

Application: WB    Species: rat    Sample:

Figure 6. |Sal upregulates the FGF2-mediated cAMP/PKA/CREB signaling pathway following MCAO/R. (A–G) Representative western blot bands of cAMP, PKA, CREB, p-CREB, FGF2 and FGFR1 in each group.

2). Hu M et al. Newly generated neurons at 2 months post-status epilepticus are functionally integrated into neuronal circuitry in mouse hippocampus. Exp Neurol 2015 Nov;273:273-87 (PubMed: 26384773) [IF=5.620]

3). Yi M et al. Exosomes secreted from osteocalcin-overexpressed endothelial progenitor cells promoted endothelial cell angiogenesis. Am J Physiol Cell Physiol 2019 Aug 14 (PubMed: 31411920) [IF=5.282]

Application: WB    Species: rat    Sample: RAOECs

Figure 3 |OCN-Exos were more effective than Exos in promoting endothelial cell angiogenesis. RAOECs treated by PBS, Exos and OCN-Exos for 24 hours were seeded on Matrigel. The representative photomicrographs of Matrigel tube formation angiogenesis assays were taken after 4, and 8 hours incubated in Matrigel-coated culture plates (A). Number (B and C) and length (D and E) of master segments were counted and measured. Protein levels of VEGFA and FGF2 were analysed by western blot. (F, G and H) Data were from four independent experiments and expressed as means ± SEM. *p<0.05, **p<0.01 vs. PBS; #p<0.05, ## p<0.05 vs. Exos. Scale bars=100 μm.

4). Iqbal M et al. Forced Physical Training Increases Neuronal Proliferation and Maturation with Their Integration into Normal Circuits in Pilocarpine Induced Status Epilepticus Mice. Neurochem Res 2019 Nov;44(11):2590-2605 (PubMed: 31560103) [IF=4.414]

5). Ren SY et al. Hair growth predicts a depression-like phenotype in rats as a mirror of stress traceability. Neurochem Int 2021 Sep;148:105110. (PubMed: 34166749) [IF=4.297]

6). Chen W et al. Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation. Biol Reprod 2018 Aug 1 (PubMed: 30084973) [IF=4.161]

7). Liu X et al. Fabrication of porous bovine pericardium scaffolds incorporated with bFGF for tissue engineering applications. Xenotransplantation 2019 Nov 6:e12568 (PubMed: 31693254) [IF=3.788]

8). Zhang M et al. Peripheral FGFR1 Regulates Myofascial Pain in Rats via the PI3K/AKT Pathway. Neuroscience 2020 Apr 8;436:1-10 (PubMed: 32278061) [IF=3.708]

Application: WB    Species: Rat    Sample: muscle cell

Fig. 3 The expression level of FGF2 was upregulated in MTrPs group. (A) The mean optical density of FGF2 in muscle cell gaps (×400). FGF2 was detected in the interstitial space of muscle cells. The level of FGF2 was significantly increased in the MTrPs group compared with that in the control group (black arrows: FGF2), n=4 rats in MTrPs and control group. **P<0.01. (B) GAPDH was used as an internal reference protein. The fold change for the density of FGF2 normalized to GAPDH was higher in the MTrPs group than that in the control group, n=6 rats in MTrPs and control group. **P<0.01.

9). Yin LL et al. Lenalidomide improvement of cisplatin antitumor efficacy on triple-negative breast cancer cells in vitro. Oncol Lett 2018 May;15(5):6469-6474 (PubMed: 29616116) [IF=3.111]

10). Zhang Z et al. Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study. PeerJ 2019 Oct 31;7:e7984 (PubMed: 31687282) [IF=3.061]

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