NIK Antibody - #DF2335
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF2335, RRID:AB_2839559.
Fold/Unfold
MAP3K14; FTDCR1B; HS; HsNIK; M3K14_HUMAN; Map3k14; Mitogen activated protein kinase kinase kinase 14; Mitogen-activated protein kinase kinase kinase 14; NF kappa beta inducing kinase; NF-kappa-beta-inducing kinase; NIK; Serine/threonine-protein kinase NIK;
Immunogens
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
- Q99558 M3K14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99558 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Ubiquitination | Uniprot | ||
| Y40 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| T559 | Phosphorylation | O15111 (CHUK) , P41279 (MAP3K8) | Uniprot |
| S643 | Phosphorylation | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| K882 | Ubiquitination | Uniprot |
PTMs - Q99558 As Enzyme
| Substrate | Site | Source |
|---|---|---|
| O14920-1 (IKBKB) | S177 | Uniprot |
| O14920-1 (IKBKB) | S181 | Uniprot |
| O15111 (CHUK) | S176 | Uniprot |
| O15111 (CHUK) | S180 | Uniprot |
| P25963 (NFKBIA) | S32 | Uniprot |
| P25963 (NFKBIA) | S36 | Uniprot |
| P61160 (ACTR2) | Y202 | Uniprot |
| P61160 (ACTR2) | T237 | Uniprot |
| P61160 (ACTR2) | T238 | Uniprot |
| Q00653-4 (NFKB2) | S865 | Uniprot |
| Q00653 (NFKB2) | S866 | Uniprot |
| Q00653-4 (NFKB2) | S869 | Uniprot |
| Q00653 (NFKB2) | S870 | Uniprot |
Research Backgrounds
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
Cytoplasm.
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Research Fields
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Intestinal immune network for IgA production. (View pathway)
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