Cleaved-Caspase 3 (Asp175), p17 Antibody

Product:	Cleaved-Caspase 3 (Asp175), p17 Antibody
Catalog:	AF7022
Description:	Rabbit polyclonal antibody to Cleaved-Caspase 3 (Asp175), p17
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat, Bovine
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	17kDa; 32kD(Calculated).
Uniprot:	P42574
RRID:	AB_2835326

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
50ul	$250	In stock
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Bovine

Prediction:

Pig(100%), Horse(86%), Sheep(100%), Rabbit(86%), Dog(100%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

Cleaved-Caspase 3 (Asp175,p17) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

RRID:

AB_2835326
Cite Format: Affinity Biosciences Cat# AF7022, RRID:AB_2835326.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Stable for 12 months from date of receipt. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A830040C14Rik; Apopain; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3, apoptosis-related cysteine peptidase; Caspase 3, apoptosis-related cysteine protease; Caspase 3, apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama;

Immunogens

Immunogen:

The antiserum was produced against synthesized peptide derived from human Caspase 3.

Uniprot:

P42574(CASP3_HUMAN) >>Visit HPA database.

Gene(ID):

CASP3(836)

Expression:

P42574 CASP3_HUMAN:

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Description:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

Sequence:

P42574 CASP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Xenopus

Rabbit

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P42574_CASP3_HUMAN

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

PTMs:

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). An orally administered gene editing nanoparticle boosts chemo-immunotherapy in colorectal cancer. Nature Nanotechnology, 2025 (PubMed: 40269250) [IF=38.1]

2). CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. Molecular Cancer, 2022 (PubMed: 35858900) [IF=37.3]

Application: WB Species: Human Sample: HepG2 and Hep3B cells

Fig. 6 FTO is regulated by circGPR137B/miR-4739 axis in HCC. A Schematic representation of the binding sites between miR-4739 and FTO 3’UTR. B Comparison of the luciferase activity of WT or Mut FTO 3’UTR after treatment with miR-4739 mimics in HepG2 and Hep3B cells. C, D RT-qPCR and western blot analysis of the expression of FTO, p21, p27, cleaved caspase-3/− 9, N-cadherin, E-cadherin and vimentin after the transfection with circGPR137B lentiviruses and (or) miR-4739 mimics in HepG2 and Hep3B cells. E, F, G MTT, colony formation and transwell analysis of the cell proliferation, colony number and cell invasion after the transfection with FTO plasmids and (or) miR-4739 mimics in HepG2 and Hep3B cells. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001

3). Microtubule polymerization induced by iridium-fullerene photosensitizers for cancer immunotherapy via dual-reactive oxygen species regulation strategy. Aggregate, 2024 [IF=18.8]

4). Prussian Blue Nanozyme Featuring Enhanced Superoxide Dismutase-like Activity for Myocardial Ischemia Reperfusion Injury Treatment. ACS Nano, 2025 [IF=16.8]

5). RIG-I, a novel DAMPs sensor for myoglobin activates NF-κB/caspase-3 signaling in CS-AKI model. Military Medical Research, 2021 (PubMed: 34148549) [IF=16.7]

6). Gene Therapy with Enterovirus 3 C Protease: A Promising Strategy for Various Solid Tumors. Nature communications, 2025 (PubMed: 40340965) [IF=16.6]

Application: WB Species: human Sample: U87 MG and U138 MG cell

Fig. 7: Ectopic expression of 3 C protease decreases the level of hnRNP A1 and induces apoptosis through cleavage of hnRNP A1. a Western blot analysis of MVP, PDIA6, hnRNP A1 and 3 C expression in U87 MG and U138 MG cells transfected with 3C-mRNA and 3 C(C147S)-mRNA and in cells transfected with 3C-mRNA and treated with AG7088. Representative blot images are shown. b The hnRNP A1 protein level decreased with increasing concentrations of 3C-mRNA. c Western blot analysis of hnRNPA1, Apaf1, Caspase-3, and cleaved-caspase-3 expression and 3 C expression in U87 MG and U138 MG cells transfected with 3C-mRNA and 3 C(C147S)-mRNA. d Western blot analysis of cleaved caspase-3, hnRNPA1, and 3 C expression in U87 MG-WT and U87 MG-hnRNP A1 KO cells transfected with 3C-mRNA and 3 C(C147S)-mRNA. Panels (a–d) are from 3 independent experiments. e Cell viability assay of U87 MG-WT and U87 MG-hnRNP A1 KO cells transfected with different concentrations of 3C-mRNA for 24 h. The data are presented as the means ± SDs. n = 3 independent biological samples. f The proposed 3 C protease–hnRNP A1–apaf-1–caspase-3–cleaved caspase-3-apoptosis axis. Source data are provided as a Source Data file.

7). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=16.6]

Application: WB Species: human Sample: HepG2

Figure 4. Effect of A22 on anti-apoptosis in 0.5 mM palmitic acid oil (PA) induced cell model. (A) Effect of A22 on cell viability for anti-apoptotic protective effect. (B) Effect of A22 on transcription of BCL-2 and BAX with measurement of mRNA levels. (C) Effect of A22 on protein expressions related with apoptosis (left), which were quantitatively analyzed (right). All the experiments were repeated for three times.

8). A Noninvasive Nanoeyedrop Therapy for the Inhibition of Uveal Melanoma: Tetrahedral Framework Nucleic Acid-Based Bioswitchable MicroRNA Delivery System. ACS Nano, 2025 [IF=15.8]

9). UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40150862) [IF=15.1]

10). Loss of Smek1 Induces Tauopathy and Triggers Neurodegeneration by Regulating Microtubule Stability. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 39206808) [IF=15.1]

Application: WB Species: Mouse Sample:

Figure 7 Smek1 deficiency caused abnormal mitochondrial transportation and function. A) Gene Ontology (GO) and B) KEGG analysis of upregulated proteins in SH-SY5Y (SMEK1-oe vs control group) proteomics. C) Electron microscope of sciatic nerve in 10-month-old mice. Red arrows pointed to microtubule disassociated to mitochondria. Scale bar, 0.2 µm. D) MitoTracker staining in SH-SY5Y cells transfected with shSMEK1 and control vector. Yellow arrows pointed to aggregated perinuclear mitochondria. Scale bar, 20 µm. E) Transfected SH-SY5Y cells were treated with lipopolysaccharide (LPS). Mitochondrial membrane potential was revealed by JC-1 staining. Scale bar, 100 µm. F) Succinodehydrogenase activity was detected in transfected NHA, HMO6, and SH-SY5Y cell lines. I) Correlation analysis of SMEK1 and mitochondria-associated gene expressions in GTEx. J,K) Annexin V-FITC and PI staining to assess apoptosis using flow cytometry in SH-SY5Y, NHA, and HMO6 cells (transfected with shNC or shSMEK1) after LPS treatment. L,M) Cortical neuron apoptosis was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) in 12-month-old mice. Scale bar, 100 µm. N = 5 per group. (N) Western blot analysis of cortices and hippocampus in 12-month-old mice. Data are mean ± SD, and are analyzed by two-sided unpaired t-test; **, p < 0.01; ****, p < 0.0001.

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Cleaved-Caspase 3 (Asp175), p17 Antibody - #AF7022