PGC1 alpha Antibody - #AF5395

Fig. 2. Peroxisome proliferator γ-activated receptor coactivator 1-α (PGC-1α) was involved in NaAsO2-induced hepatic IR and ferroptosis. (A–C) Relative PGC-1α protein and gene expression in rat livers. (D–F) The effect of PGC-1α on total and phosphorylated AKT and GSK3β expression. Cells were transfected with PGC-1α before treatment with 4 μM NaAsO2 for 48 h. Insulin was applied for 10 min before the cells were collected. (G) The effect of PGC-1α on insulin-stimulated glucose uptake. (H–J) The effect of PGC-1α on ACLS4 and GPX4 expression. (K, L) The effect of PGC-1α on Fe2+ was measured by FerroOrange staining (scale bar = 20 μm). (M, N) The effect of PGC-1α on cell lipid reactive oxygen species (ROS) measured with a lipid peroxidation fluorescent probe (scale bar = 20 μm). **P < 0.01 versus the control; #P < 0.05 and ##P < 0.01 versus the NaAsO2 (n = 3).

Fig. 3: High expression levels of S100a8/a9 inhibit viability of endothelial cells via induction of metabolic disorders. A Five types of endothelial cells were showed on the UMAP plot; B The sankey diagram showed the percentages of five types of endothelial cells in sham and CLP groups; C. The volcano map showed the noticeably upregulated genes in capillary endothelial cells; D, E The mRNA expression levels of DUSP1 detected by RT-qPCR in cell and animal experiments (n = 6 in each group); F The extent of MAPK negative feedback was evaluated by GSVA enrichment analysis; G Western blot analysis was used to assess Erk signaling pathway in lung tissues from mice (n = 6 in each group); H, I The viability of endothelial cells was evaluated by CCK8 (n = 3 in each group); J The protein expression levels of Erk signaling pathway were assessed by Western blot (n = 6 in each group); K Metabolic pathways in endothelial cells from mice were evaluated by GSVA enrichment analysis; L Heatmap showed the expression levels of mitochondrial complexes-related genes in endothelial cells from mice. M The significantly downregulated cell components were showed on the dot plot; N The protein expression levels of mitochondrial complexes were assessed by Western blot (n = 6 in each group); O Corrplot R package was used to evaluate the correlation between NRF1 and complex I-related genes in every subcluster of endothelial cell; P, Q The mRNA levels of NRF1 and NDUFA3 were measured by RT-qPCR in cell and animal experiments (n = 12 in each group), and the correlation between NRF1 and NDUFA3 was shown on the correlation curve (n = 24). Each bar showed means ± SEM. Unpaired t-test was used for the comparison between two groups. Comparison among three or more groups was analyzed by one-way ANOVA. *p 

Figure 6 Thiamine (THI) supplementation modulates energy metabolism disturbance induced by lipopolysaccharide (LPS) in RECs. (A) ATP content for different treatments. (B) The expression of AMPKα1, AMPKα2, SIRT1, and PGC1α genes with different treatments. (C) Representative Western blots for AMPKα, phosphorylated AMPKα (p-AMPKα), SIRT1, and PGC1α protein levels with different treatments. (D–G) Immunoblotting and measurement of intensity. Protein levels were normalized to the respective abundance of β-actin. Data are presented as means ± SEM (standard error of the mean), n = 3/group (results representative of at least three independent experiments); CON group, no added LPS or THI; THI group, 5 μg/mL THI; LPS group, 5 μg/mL LPS; LPTH group, 5 μg/mL THI and 5 μg/mL LPS. * denotes p < 0.05, significant difference.

Figure 6. Changes in mitochondrial-related indexes after PARK7 silencing. (A) Changes in mitochondrial synthesis proteins in APAP-treated L02 cells with and without PARK7 silencing and statistical analysis, N = 3. * p < 0.05 vs. ShNC group, ** p < 0.01 vs. ShNC group, # p < 0.05vs. ShNC+APAP group, ## p < 0.01 vs. ShNC+APAP group. (B) WB and statistical analysis of the mitochondrial synthetic proteins PGC-1α, NRF1 and TFAM in the liver tissue of mice with or without APAP intervention with PARK7 knockdown, N = 3, * p < 0.05 vs. WT group, ** p < 0.01 vs. WT group, ## p < 0.01 vs. WT+APAP group. (C,D) Changes in mitochondrial respiratory function and glycolytic function under APAP intervention with or without PARK7 silencing, N = 3. * p < 0.05 vs. shNC group, ** p < 0.01 vs. shNC group, # p < 0.05 vs. shNC+APAP group, ## p < 0.01 vs. shNC+APAP group.