BAD Antibody - #AF6471
*The optimal dilutions should be determined by the end user.
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF6471, RRID:AB_2835290.
AI325008; BAD; BAD_HUMAN; BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl-XL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; Proapoptotic BH3 Only Protein;
Expressed in a wide variety of tissues.
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q92934 As Substrate
|S75||Phosphorylation||Q9NQU5 (PAK6) , Q15349 (RPS6KA2) , Q9P1W9 (PIM2) , P31749 (AKT1) , P22612 (PRKACG) , P28482 (MAPK1) , P27361 (MAPK3) , O75582 (RPS6KA5) , Q04759 (PRKCQ) , Q13153 (PAK1) , P04049 (RAF1) , P41743 (PRKCI) , Q15418 (RPS6KA1) , P45983 (MAPK8) , Q02156 (PRKCE) , Q86V86 (PIM3) , Q15139 (PRKD1) , P17612 (PRKACA) , P22694 (PRKACB) , P51812 (RPS6KA3) , P11309 (PIM1) , O96013 (PAK4) , P15056 (BRAF) , P10398 (ARAF)||Uniprot|
|S91||Phosphorylation||P06493 (CDK1) , P45983 (MAPK8)||Uniprot|
|S99||Phosphorylation||Q9Y243 (AKT3) , P51812 (RPS6KA3) , Q04759 (PRKCQ) , Q13153 (PAK1) , P31751 (AKT2) , Q15139 (PRKD1) , P41743 (PRKCI) , P31749 (AKT1) , P15056 (BRAF) , P04049 (RAF1) , Q15418 (RPS6KA1) , Q86V86 (PIM3) , P23443 (RPS6KB1) , P17612 (PRKACA) , Q9P1W9 (PIM2) , Q02156 (PRKCE) , P11309 (PIM1) , P10398 (ARAF) , P22612 (PRKACG) , P22694 (PRKACB)||Uniprot|
|S118||Phosphorylation||Q15139 (PRKD1) , Q02156 (PRKCE) , P31751 (AKT2) , Q13153 (PAK1) , Q86V86 (PIM3) , P17612 (PRKACA) , Q9P1W9 (PIM2) , P15056 (BRAF) , Q9Y243 (AKT3) , Q15349 (RPS6KA2) , P31749 (AKT1) , Q13976 (PRKG1) , P11309 (PIM1) , P10398 (ARAF) , P04049 (RAF1) , P22612 (PRKACG) , P22694 (PRKACB) , P41743 (PRKCI) , Q15418 (RPS6KA1)||Uniprot|
|S134||Phosphorylation||Q13153 (PAK1) , P15056 (BRAF) , Q13555 (CAMK2G)||Uniprot|
|S153||Phosphorylation||P17612 (PRKACA) , O75582 (RPS6KA5) , Q15418 (RPS6KA1)||Uniprot|
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.
Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Mitochondrion outer membrane. Cytoplasm.
Note: Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm.
Expressed in a wide variety of tissues.
Forms heterodimers with the anti-apoptotic proteins, Bcl-X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity). Interacts with AKT1 and PIM3. Interacts (via BH3 domain) with NOL3 (via CARD domain); preventing the association of BAD with BCL2 (By similarity). Interacts with HIF3A (via C-terminus domain); the interaction reduces the binding between BAD and BAX (By similarity). Interacts with GIMAP3/IAN4 and GIMAP5/IAN5.
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Belongs to the Bcl-2 family.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
Application: WB Species: Human Sample: HSC-3 cells
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