BAD Antibody - #AF6471

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Product: BAD Antibody
Catalog: AF6471
Description: Rabbit polyclonal antibody to BAD
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 23kDa; 18kD(Calculated).
Uniprot: Q92934
RRID: AB_2835290

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BAD Antibody detects endogenous levels of total BAD.
RRID:
AB_2835290
Cite Format: Affinity Biosciences Cat# AF6471, RRID:AB_2835290.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI325008; BAD; BAD_HUMAN; BBC 2; BBC2; BBC6; Bcl 2 Antagonist of Cell Death; Bcl 2 Binding Component 6; BCL X / BCL 2 Binding Protein; BCL X Binding Protein; Bcl XL/Bcl 2 Associated Death Promoter; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl-XL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BCL2 antagonist of cell death protein; BCL2 associated agonist of cell death; Bcl2 Associated Death Promoter; BCL2 binding component 6; BCL2 binding protein; Bcl2 Like 8 Protein; Bcl2-L-8; BCL2L8; Proapoptotic BH3 Only Protein;

Immunogens

Immunogen:

A synthesized peptide derived from human BAD, corresponding to a region within the internal amino acids.

Uniprot:

Q92934(BAD_HUMAN) >>Visit HPA database.

Gene(ID):
BAD(572)
Expression:
Q92934 BAD_HUMAN:

Expressed in a wide variety of tissues.

Description:
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity.
Sequence:
MFQIPEFEPSEQEDSSSAERGLGPSPAGDGPSGSGKHHRQAPGLLWDASHQQEQPTSSSHHGGAGAVEIRSRHSSYPAGTEDDEGMGEEPSPFRGRSRSAPPNLWAAQRYGRELRRMSDEFVDSFKKGLPRPKSAGTATQMRQSSSWTRVFQSWWDRNLGRGSSAPSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

PTMs:

Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis.

Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.

Subcellular Location:

Mitochondrion outer membrane. Cytoplasm.
Note: Colocalizes with HIF3A in the cytoplasm (By similarity). Upon phosphorylation, locates to the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a wide variety of tissues.

Family&Domains:

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

Belongs to the Bcl-2 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

References

1). The anti-hepatocellular carcinoma effect of Brucea javanica oil in ascitic tumor-bearing mice: The detection of brusatol and its role. Biomedicine & Pharmacotherapy, 2021 (PubMed: 33341052) [IF=6.9]
2). MicroRNA-27b alleviates septic cardiomyopathy by targeting the Mff/MAVS axis. Frontiers in cellular and infection microbiology, 2025 (PubMed: 40766842) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 5 Mff overexpression reverses the protective effects of miR-27b against inflammation and apoptosis. (B–D), ELISA detection of IL-1β, IL-6, and TNF-α levels in each cell group. (A, E, F), Western blot quantitative analysis of Bad and Bax protein expression. (G), CCK-8 detection of cell viability in each group. (A, H, I), Quantitative analysis of Bcl-2 and XIAP protein expression. (J), Quantitative analysis of MAVS protein expression. (K), Immunofluorescence staining detecting MAVS expression and localization, scale bar=50 μm. Data are presented as mean ± s.d. (n=3 independent experiments). #P
3). LT-α Facilitates the Aerobic Glycolysis and M1 Polarization of Macrophages by Activating the NF-κB Signaling Pathway in Intervertebral Disc Degeneration. Journal of inflammation research, 2025 (PubMed: 40125079) [IF=4.5]
4). Griffithazanone A, a sensitizer of EGFR-targeted drug in Goniothalamus yunnanensis for non-small cell lung cancer. Heliyon, 2024 (PubMed: 39403494) [IF=4.0]

Application: WB    Species: human    Sample: A549 cells

Fig. 3 Griffithazanone A targeted PIM1, regulated downstream ASK1/JNK/p38 and BAD/Bcl-2 pathways, and promoted apoptosis in A549 cells. (a) The docking stick model of griffithazanone A combining with the PHE-254/PRO-241/GLU-247/GLN-252/ARG-250 domain of PIM1. Molecular docking score achieved −7.69. (b, c) Cellular thermal shift assay (CETSA): Used PBS as a negative control, quantitatively analyzed of the binding of griffithazanone A and PIM1 in A549 cells at different temperatures, and evaluated the expression using grayscale analysis. (d–g) Western blot analysis: p-ASK1, ASK1, p-JNK, JNK, p-p38, p38, p-BAD, BAD, p-foxo3a, foxo3a, Bax, Bcl-2, caspase 3 and cleaved-caspase 3 expression and its gray level in A549 cells. Take Tubulin as the internal parameter. (h) qRT-PCR showed the mRNA expression levels of Bax and Bcl-2 in A549 cells. (i) A549 cells were incubated with griffithazanone A (0.5 1 and 2 μM), and then detect the ROS level using a fluorescence enzyme-linked immunosorbent assay.
5). Effective extraction of Xuetongsu and its role in preventing RA synovial hyperplasia by targeting synovial cell migration and apoptosis. Scientific reports, 2024 (PubMed: 39375373) [IF=3.8]
6). Study on the Mechanism of Sancao Tiaowei Decoction in the Treatment of MNNG-Induced Precancerous Lesions of Gastric Carcinoma Through Hedgehog Signaling Pathway. Frontiers in Oncology, 2022 (PubMed: 35646631) [IF=3.5]
7). Anlotinib suppresses oral squamous cell carcinoma growth and metastasis by targeting the RAS protein to inhibit the PI3K/Akt signalling pathway. Analytical Cellular Pathology, 2021 (PubMed: 34926131) [IF=2.6]

Application: WB    Species: Human    Sample: HSC-3 cells

Figure 6 Effect of anlotinib on the RAS and PI3K/Akt signalling pathways in HSC-3 cells. HSC-3 cells were treated with anlotinib for 72 hours. Total RAS, phosphorylated and total Akt, and β-actin were detected by western blotting. (a) Molecular mechanism of anlotinib in HSC-3 cell apoptosis. (b) Changes in the p-Akt/Akt ratio in apoptosis. (c) Ratio changes of RAS, PI3K, and Bad proteins in apoptosis based on quantitative results of protein expression. The data are expressed as the mean ± standard deviation of three repeats. ∗∗∗P < 0.001 compared with the control.
8). Downregulated lncRNA HOTAIR ameliorates polycystic ovaries syndrome via IGF-1 mediated PI3K/Akt pathway. Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, 2023 (PubMed: 37356454) [IF=2.0]
9). Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway. Journal of visualized experiments : JoVE, 2024 (PubMed: 39248530) [IF=1.2]

Application: WB    Species: rat    Sample: renal tissue

Figure 4: Kemeng Fang alleviates podocyte damage by activating the PI3K/AKT signaling pathway. (A-C) IHC was used to detect the relative expression levels of two podocyte marker proteins, WT-1, and Nephrin, in renal tissue. (D-E) PCR detection of the relative mRNA expression of two podocyte marker proteins, WT-1 and Nephrin, in renal tissue. (F-G) TUNEL staining was used to detect the incidence of apoptosis in renal tissue. (H) Observation of glomerular basement membrane and podocyte mitochondrial structure using TEM (2,500x, bar=5 µM; 7,000x, bar=2 µM). (I-J) WB detection of relative protein expression levels of PI3K, PIK3CA, AKT, P-AKT, BAD, P-BAD, BCL-2, bax, and C-caspase3 in renal tissue.

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